PXD065837-1

PXD065837 is an original dataset announced via ProteomeXchange.

Title	Sulfoxide-diazirine (SODA) as a cleavable photoaffinity group to enable identification of photolabeled modification sites via LC-MS3
Description	Photoaffinity labeling is a common approach in chemical proteomics for identification of small molecule probe tar-gets. However, the modification site on the target protein remains difficult to identify, because of the probe modifica-tion and potential fragmentation during tandem mass spectrometry. We here introduce MS-cleavable photoaffinity groups for a better identification of the modification site. These are based on diazirine photoreactive groups and sul-foxide as MS-labile linker. We have applied these in photoaffinity probes based on the peptide-like aspartic protease inhibitor pepstatin A, and we demonstrate that we can identify and map binding hotspots on structural models of a target protease.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-25_07:09:10.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Verhelst
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 01:06:48	ID requested
⏵ 1	2025-08-25 07:09:10	announced

10.1021/ACS.ANALCHEM.5C02409;

submitter keyword: Sulfoxide-diazirine, photolabeleing, LC-MS3, cleavable photoaffinity group

Steven Verhelst
contact affiliation	Laboratory of Chemical Biology, Department of Cellular and Molecular Medicine, KU Leuven – University of Leuven
contact email	steven.verhelst@kuleuven.be
lab head
Steven Verhelst
contact affiliation	KU Leuven
contact email	steven.verhelst@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065837
     1. Label: PRIDE project
     2. Name: Sulfoxide-diazirine (SODA) as a cleavable photoaffinity group to enable identification of photolabeled modification sites via LC-MS3