PXD065801-1

PXD065801 is an original dataset announced via ProteomeXchange.

Title	Site-Specific N-Glycosylation Alterations in Serum Proteins Across Subtypes of Adolescent Depressive Disorder
Description	Background: Depressive disorder in adolescents is a clinically heterogeneous condition with poorly defined molecular underpinnings. Glycosylation, a key post-translational modification involved in immune and neuronal regulation, has not been systematically studied across depressive subtypes in youth. Methods: We employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with site-specific glycoproteomic profiling to analyze serum samples from adolescents diagnosed with major depressive disorder, stratified into three subtypes: depression, depression with non-suicidal self-injury (NSSI), and depression with suicide attempts. Healthy adolescents served as controls. Intact N-glycopeptides were quantified to identify subtype-specific glycosylation patterns. Results: Distinct alterations in serum N-glycosylation were observed across depressive subtypes. All groups exhibited decreased levels of bi-antennary glycans and LacNAc/Lewis structures, alongside elevated expression of tetra-antennary sialylated glycans, particularly N6H7S4. NSSI cases showed increased tri-antennary sialylation, while suicidal depression was marked by specific upregulation of N6H7S4 on ORM2. Differentially glycosylated proteins, including SERPING1 and A2M, were enriched in immune, complement, and coagulation pathways. These changes occurred independently of total protein abundance, indicating glycan-specific dysregulation. Conclusion: Our findings uncover subtype-dependent N-glycosylation signatures in adolescent depression and implicate glycan-mediated immune and inflammatory mechanisms in disease heterogeneity. Glycoproteomic profiling offers new insights into biomarker discovery and the molecular classification of depressive subtypes.
HostingRepository	iProX
AnnounceDate	2025-07-03
AnnouncementXML	Submission_2025-07-04_23:52:25.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinyuan Hu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-04 23:52:10	ID requested
⏵ 1	2025-07-04 23:52:25	announced

Dataset with its publication pending

submitter keyword: Adolescent depressive disorder

Glycoproteomics

N-glycosylation;Biomarkers

Immune regulation

Xinyuan Hu
contact affiliation	Department of Psychiatry, Shandong Mental Health Center, Shandong University
contact email	moonriveryue@163.com
lab head
Xinyuan Hu
contact affiliation	Department of Psychiatry, Shandong Mental Health Center, Shandong University
contact email	moonriveryue@163.com
dataset submitter

iProX dataset URI