PXD065795-1

PXD065795 is an original dataset announced via ProteomeXchange.

Title	Proteomic Sensors for Quantitative, Multiplexed, and Spatial Monitoring of Kinase Signaling
Description	Proteomic Kinase Activity Sensors (ProKAS) is a new technology developed to allow for quantitative and multiplexed analyses of kinase signaling in living cells with spatial resolution. Kinase sensors are expressed in cells as part of a ProKAS construct, which uses signal peptides to localize to specific subcellular regions. The kinase sensors are targets for phosphorylation by specific kinases due to their amino acid sequences, which are derived from phosphoproteomic data or designed de novo using PSPA data that indicates kinase motif preferences. Includes data generated for revisions.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_12:20:43.648.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	William Comstock
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	6x(13)C; 6x(13)C; phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-04 11:42:24	ID requested
⏵ 1	2025-10-06 12:20:44	announced

Dataset with its publication pending

submitter keyword: Proteomics ATR ATM CHK1 DNA Damage Genome Maintenance Signaling Phosphoproteomics PRM

Marcus Smolka
contact affiliation	Cornell University, Ithaca, NY, USA
contact email	mbs266@cornell.edu
lab head
William Comstock
contact affiliation	Cornell University
contact email	wc622@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD065795

PRIDE project URI

• PRIDE
  1. PXD065795
     1. Label: PRIDE project
     2. Name: Proteomic Sensors for Quantitative, Multiplexed, and Spatial Monitoring of Kinase Signaling