PXD065794-1

PXD065794 is an original dataset announced via ProteomeXchange.

Title	Sac1 degron A431 cells 3h DMSO vs 3h IAA
Description	Sac1 is a conserved phosphoinositide phosphatase, whose loss-of-function compromises cell and organism viability. Here, we employed acute auxin-inducible Sac1 degradation to identify its immediate downstream effectors in human cells. Most of Sac1 was is degraded in ~1 h, paralleled by increased PI(4)P and decreased cholesterol in the trans-Golgi network (TGN) during the following hour, and superseded by Golgi fragmentation, impaired glycosylation, and selective degradation of TGN proteins by ~4 h. The TGN disintegration resulted from its acute deacidification caused by disassembly of the Golgi V-ATPase. Mechanistically, Sac1 mediated TGN membrane composition maintained an assembly-promoting conformation of the V0a2 subunit. Key phenotypes of acute Sac1 degradation were are recapitulated in human differentiated trophoblasts, causing processing defects of chorionic gonadotropin, in line with loss-of-function intolerance of the human SACML1 gene. Collectively, our findings reveal that the assembly of the Golgi V-ATPase is controlled by the TGN membrane via Sac1 fuelled lipid exchange.
HostingRepository	PRIDE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-08_02:57:28.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miesje van der Stoel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-04 11:36:33	ID requested
⏵ 1	2025-07-08 02:57:28	announced

Dataset with its publication pending

submitter keyword: human A431 cells, Sac1, auxin inducible degradation

Elina Ikonen
contact affiliation	Stem Cells and Metabolism Research Program and Dept. of Anatomy, Faculty of Medicine, University of Helsinki, Helsinki, Finland; Minerva Foundation Institute for Medical Research, Helsinki, Finland;
contact email	elina.ikonen@helsinki.fi
lab head
Miesje van der Stoel
contact affiliation	University of Helsinki
contact email	miesje.vanderstoel@helsinki.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD065794

PRIDE project URI

• PRIDE
  1. PXD065794
     1. Label: PRIDE project
     2. Name: Sac1 degron A431 cells 3h DMSO vs 3h IAA