PXD065788-1

PXD065788 is an original dataset announced via ProteomeXchange.

Title	A post-translational proteomic survey of a single anatomically preserved human 20-week post-conception brain
Description	Progress in defining the proteome of the developing human brain has lagged behind our understanding of the adult human brain, primarily due to challenges in tissue acquisition and in preservation of anatomical structure during experimental processing. Upon receipt of a single, exceptionally well-preserved 20 post-conception week human brain hemisphere, we conducted fine dissections of 18 anatomically distinct brain regions, including the pia mater. These samples underwent in-depth analysis of both the total and post-translationally modified proteomes, with the aim of creating a reference resource for investigators studying this critical stage of neurodevelopment. Here, we present an overview of the resulting dataset, compare the proteomic profiles across regions, and highlight examples of variable post-translational modifications within individual proteins to guide data interpretation. As expected, unmodified protein profiles reveal substantial differences across brain regions. For instance, pia mater and thalamus are enriched for proteins involved in transcription and chromatin organization, consistent with a higher proportion of dividing cells in these areas at this developmental stage. In contrast, the cortical proteome reflects active synaptogenesis and cytoskeletal remodelling. While inter-regional differences in phosphorylated and acetylated peptides largely mirror those observed in the unmodified proteome with respect to gene ontology categories, the glycosylated peptidome of the pia mater is markedly distinct. This divergence is driven by the secretion of extra-cellular matrix proteins and the region’s intimate association with basement membrane of the pia. Finally, by integrating our proteomic data with publicly available single- nucleus RNA sequencing datasets from the same developmental stage, we identify high-confidence ligand receptor pairs (e.g., L1CAM:CD9,CNTN4:PTPRG, LGALS1:ITGB1) likely involved in thalamocortical signalling.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_17:04:24.694.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pia Jensen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; deglycosylated asparagine; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-04 05:10:48	ID requested
⏵ 1	2026-09-06 17:04:25	announced

10.1111/joa.70170;

Bandiera S, Bogetofte H, Jensen P, Hussain R, Nischal SA, Rihova L, Clowry GJ, Larsen MR, Moln, á, r Z, Carlyle BC, A posttranslational proteomic survey of a single anatomically preserved human 20-week postconception brain. J Anat, ():(2026) [pubmed]

submitter keyword: human brain development, post-translational modifications, TMT,Proteomics, Exploris 480

Becky Carlyle
contact affiliation	ARUK Senior Research Fellow & Departmental Research Lecturer Department of Physiology, Anatomy and Genetics, Kavli Institute for Nanoscience Discovery, University of Oxford, UK
contact email	becky.carlyle@dpag.ox.ac.uk
lab head
Pia Jensen
contact affiliation	University of Southern Denmark
contact email	pjensen@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD065788

PRIDE project URI

• PRIDE
  1. PXD065788
     1. Label: PRIDE project
     2. Name: A post-translational proteomic survey of a single anatomically preserved human 20-week post-conception brain