PXD065786 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HDX-MS of Human Histidine Rich Glycoprotein (HRG) |
| Description | Histidine rich glycoprotein (HRG) is a multi-domain plasma glycoprotein, which is implicated in numerous biological processes including immune regulation, angiogenesis, coagulation and fibrinolysis. Despite a diverse role of biological functions, structural investigations into HRG have yielded only limited information, and there are no 3-dimensional structures of the intact protein. Current computational modelling suggests that HRG exhibits a large degree of conformational flexibility, which likely contributes to its well-documented multifunctionally. Here, utilising amide hydrogen-deuterium exchange mass spectrometry (HDX-MS), and combining insights from AlphaFfold, we investigate the conformational landscape of HRG. Overall, our data reveal a high degree of H/D exchange within the HRR, PRR and C terminal domains, indicating these regions are exposed to the aqueous environment surrounding the HRG surface, and provides the strongest evidence yet that these are largely disordered in vivo. Overall, this investigation lays the foundation for understanding the conformational architecture of HRG and paves the way for future studies aimed at elucidating how the interactions between HRG and other molecules are mediated, potentially revealing new mechanisms of its biological activity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-06 |
| AnnouncementXML | Submission_2025-11-06_05:26:27.942.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Glenn Masson |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | phosphorylated residue |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-04 04:38:41 | ID requested | |
| ⏵ 1 | 2025-11-06 05:26:28 | announced | |
Publication List
| Hierons SJ, Lin B, Fritzen R, Blindauer CA, Ajjan RA, Masson GR, Stewart AJ, Investigating the conformational landscape of human histidine-rich glycoprotein using amide HDX-MS. Biochem J, 482(22):1721-35(2025) [pubmed] |
| 10.1042/bcj20253366; |
Keyword List
| ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
| submitter keyword: HRG,HDX-MS |
Contact List
| Glenn Masson |
|---|
| contact affiliation | University of Dundee |
| contact email | gmasson001@dundee.ac.uk |
| lab head | |
| Glenn Masson |
|---|
| contact affiliation | University of Dundee |
| contact email | gmasson001@dundee.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD065786 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065786
- Label: PRIDE project
- Name: HDX-MS of Human Histidine Rich Glycoprotein (HRG)
