PXD065755-1

PXD065755 is an original dataset announced via ProteomeXchange.

Title	The interaction of microplastics with the ruminal ecosystem in vitro
Description	Mismanagement of plastic waste has contributed to plastic pollution in marine and terrestrial ecosystems. Therefore, farm animals are likely to consume microplastic (MP) contaminated feed. However, the interactions of MP with the ruminal microbial ecosystem remain poorly understood. This study investigated the interaction of MP within the ruminal ecosystem in vitro using the Hohenheim Gas Test. Different MP variants were applied, reflecting different combinations of five MP species (polylactide, polyhydroxy butyric acid, high-density polyethylene, polyvinyl chloride, polypropylene), in two particle size ranges (<125 μm; 125-500 μm) and increasing dosages (from 0 to 70 mg /incubation cylinder) together with ruminal fluid and hay or barley as substrates. Cumulative gas production, pH and dry matter disappearance were determined before analyzing volatile fatty acids, metaproteomics and metabolomics. In the presence of MP, cumulative gas production decreased regardless of the MP 63 species, dose or particle size, while total dry matter degradation increased. Microbial proteins in barley incubations showed lower Bacteroidetes and increasing Firmicutes abundance in the presence of MP and increased activities of the protein groups `replication and repair`, and `translation`, but decreased activities of `carbohydrate metabolism and transport` and `amino acids metabolism`. The data indicated that MP, regardless of their species and particle size, interact with the ruminal microbiome and may be partially degraded in vitro. This suggests a reduction of MP size in the rumen thereby increasing the likelihood to penetrate animal tissues. Future research must confirm the findings in vivo and determine their consequences for animal health and consumer safety.
HostingRepository	PRIDE
AnnounceDate	2026-03-28
AnnouncementXML	Submission_2026-03-28_00:28:53.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Seifert
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 09:54:04	ID requested
⏵ 1	2026-03-28 00:28:54	announced

10.1016/j.jhazmat.2025.140481;

Eichinger J, Seifert J, S, á, enz JS, Amin N, Lorenz S, Eckel F, Zollfrank C, Windisch W, Brugger D, The interaction of microplastics with the ruminal ecosystem in vitro. J Hazard Mater, 500():140481(2025) [pubmed]

submitter keyword: metaproteomics, microbiome, microplastic, rumen

Prof. Dr Jana Seifert
contact affiliation	University of Hohenheim, Functional Microbiology of Livestock, Stuttgart, Germany
contact email	jseifert@uni-hohenheim.de
lab head
Jana Seifert
contact affiliation	University of Hohenheim
contact email	jseifert@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065755
     1. Label: PRIDE project
     2. Name: The interaction of microplastics with the ruminal ecosystem in vitro