PXD065753-1

PXD065753 is an original dataset announced via ProteomeXchange.

Title	cJUN dependent innate immunity controls microbiome through selective phagocytosis in Nematostella
Description	Innate immunity, traditionally considered as a broad and non-specific defense system, is increasingly recognized for its capacity to selectively regulate microbial communities. Emerging evidence suggests that invertebrates can differentiate between closely related microorganisms, challenging the dichotomy between innate and adaptive immunity. This specificity is crucial for maintaining symbiotic relationships while preventing colonization by harmful microbes. In the sea anemone Nematostella vectensis, nematosomes—motile immune cells—play a key role in selective phagocytosis, preferentially engulfing foreign Vibrio isolates while sparing native bacterial colonizers. Here, wWe identifiedy the transcription factor cJUN as a crucial regulator of nematosome proliferation and function. CRISPR/Cas9-mediated knockout of cJUN resulted in a significant reduction of nematosome proliferation, impaired lysosomal activation, and an altered microbiome composition with increased colonization of non-native Vibrio strains. These findings demonstrate a direct link between host immune specificity and microbial community structure, challenging the traditional view of innate immunity as a non-specific defense. Our study highlights the evolutionary conservation of selective innate immune mechanisms and their role in maintaining microbial homeostasis in early-branching metazoans.
HostingRepository	PRIDE
AnnounceDate	2026-07-24
AnnouncementXML	Submission_2026-07-24_03:08:04.361.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Nematostella vectensis; NCBI TaxID: NEWT:45351;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 09:30:45	ID requested
⏵ 1	2026-07-24 03:08:04	announced

10.1038/S41467-026-75511-W;

submitter keyword: phagocytosis,LC-MS, Nematostella vectensis, proteomics, microbiome, nematocomes, immunity, label-free

Prof. Dr. Andreas Tholey
contact affiliation	Institut für Experimentelle Medizin (IEM) Arbeitsgruppe Systematische Proteomforschung Christian-Albrechts-Universität zu Kiel Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065753
     1. Label: PRIDE project
     2. Name: cJUN dependent innate immunity controls microbiome through selective phagocytosis in Nematostella