PXD065743-1

PXD065743 is an original dataset announced via ProteomeXchange.

Title	Integrating Cell Painting and Thermal Proteome Profiling for Inference of Targets and Mechanism of Action
Description	Understanding the mechanism of action (MoA) of bioactive compounds is a central challenge in drug discovery and chemical biology. In this article, we propose a strategy for integrating morphological data with proteomics to provide deeper insights into the MoA of compounds. We combine the rich phenotypic profiles of Cell Painting (CP) with the unbiased protein target detection of Thermal Proteome Profiling (TPP), and construct protein–protein interaction networks based on potential targets identified using both assays. We evaluate our method on public TPP data sets for the five compounds (+)-JQ1, I-BET151, Vemurafenib, Crizotinib and Panobinostat, together with Cell Painting data for 5270 drugs on U2OS cells. We show that the combined approach could accurately identify known MoAs for four out of five compounds. This work highlights the value of multimodal profiling for chemical biology and opens new avenues for data-driven discovery of therapeutic mechanisms.
HostingRepository	PRIDE
AnnounceDate	2025-12-18
AnnouncementXML	Submission_2025-12-18_05:21:02.153.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mårten Larsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 07:44:21	ID requested
⏵ 1	2025-12-18 05:21:02	announced

Dataset with its publication pending

submitter keyword: thermal proteome profiling, mechanism of action, drug target,cell painting

Erik T. Jansson
contact affiliation	Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
contact email	erik.jansson@uu.se
lab head
Mårten Larsson
contact affiliation	Uppsala University
contact email	marten.larsson@imbim.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD065743

PRIDE project URI

• PRIDE
  1. PXD065743
     1. Label: PRIDE project
     2. Name: Integrating Cell Painting and Thermal Proteome Profiling for Inference of Targets and Mechanism of Action