PXD065739-1

PXD065739 is an original dataset announced via ProteomeXchange.

Title	A Proximity dependent labeling approach to define flotillin-2 interacting proteins within Membrane Domains of HeLa cells
Description	The formation of plasma membrane microdomains, known as lipid rafts, is driven by specific interactions among cholesterol, sphingolipids, and certain proteins such as flotillins. Flotillins are known to oligomerize and associate with defined lipid assemblies, thereby promoting the coalescence and stabilization of these membrane rafts. Acting as scaffolding molecules, flotillins play critical roles in various cellular processes, including the spatial organization of signaling complexes and membrane compartmentalization. To identify flotillin-associated proteins within membrane rafts in vivo, we employed the BioID proximity labeling technique to analyze the flotillin-2 interactome in living HeLa cells. Flotillin-2 was fused to the promiscuous biotin ligase BirA* at either the N- or C-terminus and expressed in HeLa cells, enabling biotinylation of proximal proteins. Biotinylated proteins were enriched on streptavidin affinity beads and subsequently identified via LC-MS analysis. This investigation of the flotillin-2 interactome provides new insights into the molecular composition of membrane rafts and offers a valuable framework for understanding the mechanisms underlying raft formation, regulation, and associated cellular functions in native membranes.
HostingRepository	PRIDE
AnnounceDate	2025-08-22
AnnouncementXML	Submission_2025-08-22_04:39:08.734.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065739
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Tracz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 06:56:40	ID requested
⏵ 1	2025-08-22 04:39:09	announced

10.6019/PXD065739;

10.1016/J.DIB.2025.111970;

submitter keyword: Flotillin-2 FLOT2 PPI protein-protein interactions BioID proximity labelling DRM lipid rafts HeLa

Michał Tracz
contact affiliation	Mass Spectrometry Laboratory, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliation	Laboratory of Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065739
     1. Label: PRIDE project
     2. Name: A Proximity dependent labeling approach to define flotillin-2 interacting proteins within Membrane Domains of HeLa cells