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PXD065721-1

PXD065721 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIDENTIFICATION AND CHARACTERISATION OF TAXONOMICALLY INFORMATIVE PEPTIDES BY HIGH RESOLUTION MASS SPECTROMETRY FOR SOURCE IDENTIFICATION OF TRACE HAIR
DescriptionIn this study a proteomics workflow was developed for the discovery and characterization of taxonomically diagnostic hair peptides. Taxa were selected with a focus on relevance to crime scene investigation. A panel of 226 taxonomically informative peptides were identified and characterized, 209 which appear novel to this study. This included 59 peptides with single-species specificity, while the remainder were diagnostic due to restricted or convergent taxonomic distribution. The panel of taxa-discriminating markers was subsequently integrated into a forensic workflow. The developed workflow was used to analyze single fur-hair (20 mm), from four individuals from each species of interest. The data was processed using a curated protein database, providing an efficient and validated bioinformatics workflow that did not require prior source information. The method was able to classify all hairs analyzed with genus or species level resolution.
HostingRepositoryPRIDE
AnnounceDate2026-05-13
AnnouncementXMLSubmission_2026-05-13_10:53:57.612.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRomy Keane
SpeciesList scientific name: Felis catus (Cat) (Felis silvestris catus); NCBI TaxID: NEWT:9685; scientific name: Canis lupus; NCBI TaxID: NEWT:9612; scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; scientific name: Cavia porcellus (Guinea pig); NCBI TaxID: NEWT:10141; scientific name: Sus scrofa (Pig); NCBI TaxID: NEWT:9823; scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: NEWT:9986; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; scientific name: Vicugna pacos; NCBI TaxID: NEWT:30538; scientific name: Camelus dromedarius (Dromedary) (Arabian camel); NCBI TaxID: NEWT:9838;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Exploris 240
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-07-03 02:17:52ID requested
12026-05-13 10:53:58announced
Publication List
10.1021/ACS.JPROTEOME.5C00638;
Keyword List
submitter keyword: Taxonomically informative biomarkers, hair proteins, forensic proteomics, trace evidience
Contact List
Joel P A Gummer
contact affiliationChemCentre, Forensic Science Laboratory, Building 500, Curtin University, Bentley, WA, Australia, 6102
contact emailjgummer@chemcentre.wa.gov.au
lab head
Romy Keane
contact affiliationChemcentre
contact emailr.keane@ecu.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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