PXD065702-1

PXD065702 is an original dataset announced via ProteomeXchange.

Title	Two opposing redox signals mediated by 2-Cys peroxiredoxin shape the redox proteome during photosynthetic induction
Description	Photosynthetic induction, characterized by the lag in CO2 assimilation rates during transition from darkness to light, has traditionally been attributed to Rubisco activase activity and stomatal opening. Yet, the faster induction of photosynthesis in the 2-Cys peroxiredoxins (Prxs) mutant (2cpab) suggested a role for oxidative signals in regulating photosynthetic rates, although the underlying molecular mechanism remains unclear. SPEAR, a redox proteomics approach, was used to systematically map redox changes occurring during photosynthesis induction and to unravel the role of 2-Cys Prxs in shaping these redox alterations. No significant difference was observed in protein expression levels between WT and 2cpab plants, suggesting that protein abundance does not account for the 2cpab phenotype. During the transition from dark to low light, 82 and 54 cysteine-containing peptides were reduced or oxidized, respectively, in WT plants. Most redox-regulated cysteines in photosynthetic proteins were found oxidized in the dark and became reduced in response to light. A reverse pattern was observed among redox-regulated cysteines in proteins involved in starch degradation and chloroplast glycolysis, which shifted from a reduced to an oxidized state in response to light. These findings demonstrate the initiation of two opposing redox responses, affecting distinct sets of metabolic proteins during the induction phase. Remarkably, a significantly lower number of cysteines were reduced or oxidized in 2cpab plants, highlighting the crucial role 2-Cys Prxs play in shaping both signals. Taken together, rotational shifts between metabolic pathways during the photosynthesis induction phase are regulated by two opposing redox signals mediated by 2-Cys Prx activity.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_17:46:21.376.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alon Savidor
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	N-ethylmaleimide derivatized cysteine; hydrolyzed N-ethylmaleimide cysteine adduct
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-02 18:35:09	ID requested
⏵ 1	2025-08-24 17:46:22	announced

10.1016/j.redox.2025.103810;

Doron S, Lampl N, Savidor A, Pri-Or A, Katina C, Cejudo FJ, Levin Y, Rosenwasser S, Two opposing redox signals mediated by 2-cys peroxiredoxin shape the redox proteome during photosynthetic induction. Redox Biol, 86():103810(2025) [pubmed]

submitter keyword: photosynthesis induction,Redox proteomics, LC-MS, redox signaling, SPEAR, 2-Cys peroxiredoxins

Shilo Rosenwasser
contact affiliation	The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew Universityof Jerusalem, Rehovot 7610001, Israel.
contact email	shilo.rosenwaser@mail.huji.ac.il
lab head
Alon Savidor
contact affiliation	The Weizmann Institute of Science
contact email	alon.savidor@weizmann.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065702
     1. Label: PRIDE project
     2. Name: Two opposing redox signals mediated by 2-Cys peroxiredoxin shape the redox proteome during photosynthetic induction