PXD065672-1

PXD065672 is an original dataset announced via ProteomeXchange.

Title	DNA polymerase iota alleviates replication stress-induced genome instability during mitosis
Description	DNA polymerase iota (Pol ι) is a Y-family lesion bypass polymerases, characterized by its remarkably low fidelity. While Pol ι is ubiquitously expressed in nearly all cell types, its function remains largely undefined. Previous studies in cellular and mouse models deficient in Pol ι have revealed no discernible phenotype. In this report, we show that Pol ι plays an important role in mitotic DNA synthesis. Cells lacking Pol ι exhibit heightened vulnerability to replication stress, increased formation of micronuclei, and accumulation of chromosomal breaks. The protein level of Pol ι is tightly regulated and restricted to G2/M phase cells via CRL7/FBXW11-mediated proteasomal degradation. The absence of Pol ι during S phase ensures that its error-prone DNA synthesis activity is excluded from interfering with normal DNA replication. These findings suggest that Pol ι function is critical in resolving DNA damage-induced replication stress prior to the completion of mitosis.
HostingRepository	iProX
AnnounceDate	2025-07-01
AnnouncementXML	Submission_2025-07-01_20:38:48.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Can
SpeciesList	scientific name: Human parechovirus strain T82-0169; NCBI TaxID: 1001056;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-01 20:38:29	ID requested
⏵ 1	2025-07-01 20:38:48	announced

Dataset with its publication pending

submitter keyword: Biological sciences, Cell biology, Cell division/DNA replication, Translesion synthesis Biological sciences/Molecular biology, DNA damage and repair, DNA damage response

Lei Li
contact affiliation	Zhejiang University
contact email	leili2020@zju.edu.cn
lab head
Yi Can
contact affiliation	Zhejiang University
contact email	yican1998@zju.edu.cn
dataset submitter

iProX dataset URI