PXD065585-1

PXD065585 is an original dataset announced via ProteomeXchange.

Title	AP2X-1 is a negative regulator of Toxoplasma gondii sexual commitment
Description	Toxoplasma gondii is a widespread protozoan with a complex life cycle, characterized by transitions between various hosts and developmental stages, each tailored to a specific niche within its host. However, the regulatory mechanisms governing these life cycle transitions are not well understood. In this study, we investigated the AP2 factor AP2X-1, which is expressed during the tachyzoite and bradyzoite stages but decreases in the mature merozoite stage. Knockout of ap2X-1 significantly impaired tachyzoite invasion and replication while increasing the frequency of bradyzoite differentiation. As a component associated with the HDAC3/MORC complex, loss of ap2X-1 led to the upregulation of bradyzoite- and sexual stage-specific genes. Single-cell sequencing revealed that ap2X-1 knockout strain exhibited a mixed population of tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like parasites. CUT&Tag analysis revealed a substantial overlap between AP2X-1 and HDAC3/MORC complex at the promoters of bradyzoite- and sexual stage-specific genes. Additionally, ATAC-seq analysis demonstrated that AP2X-1 influences chromatin compaction and accessibility, suggesting that AP2X-1 may modulate the function of the HDAC3/MORC complex to facilitate the repression of bradyzoite differentiation and sexual commitment. Loss of ap2X-1 resulted in a significant attenuation of T. gondii virulence and decreased brain cyst formation in vivo. These findings identify AP2X-1 as a critical negative regulator of T. gondii sexual development.
HostingRepository	PRIDE
AnnounceDate	2025-06-29
AnnouncementXML	Submission_2025-06-28_23:16:59.226.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JinLei Wang
SpeciesList	scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-28 21:04:49	ID requested
⏵ 1	2025-06-28 23:16:59	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii

AP2X-1

HDAC3/MORC complex

gene expression

sexual development

transcription

Jin-Lei Wang
contact affiliation	State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
contact email	wangjinlei90@126.com
lab head
JinLei Wang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
contact email	wangjinlei90@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD065585

PRIDE project URI

• PRIDE
  1. PXD065585
     1. Label: PRIDE project
     2. Name: AP2X-1 is a negative regulator of Toxoplasma gondii sexual commitment