PXD065581-1

PXD065581 is an original dataset announced via ProteomeXchange.

Title	Identification of serum protein biomarkers for pre-cancerous lesions associated with Pancreatic Ductal Adenocarcinoma (Part3:Human)
Description	Pancreatic ductal adenocarcinoma (PDAC) has poor prognosis as early-stage asymptomaticity leads to late-stage diagnoses. Strategies to detect PDAC earlier or identify predisposed individuals are therefore paramount. Here we report results from genetically engineered mice (GEMMs) and PDAC patients that identify serum proteins associated with pancreatic intraepithelial neoplasms (PanINs) (the most common PDAC precursor) and early-stage PDAC. Initially we screened previously described PanIN-abundant GEMMs, harbouring Pdx1-Cre, Lox-STOP-Lox-KrasG12D/+ and floxed alleles of essential autophagy genes Atg7-/- or Atg5-/-. Serum from these mice was assessed by proteomics and hits compared to those identified in KrasG12D/+ Trp53R172H/+ Pdx1-Cre (KPC) mice (which closely recapitulate human disease) and early-stage (I-II) PDAC patients. Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) was elevated in all three screens, with complement C5, complement factors B and H, and CD14 increased in KPC mice and PDAC patients; all significantly increase co-ordinately in PDAC, with each elevating with disease stage. Serum levels of C5, CFH and CD14 together constitute a novel panel for identifying PanINs and early-stage PDAC with confidence, and when combined with additional screening could help increase survival from this significant disease.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_19:31:43.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Lilla
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-28 17:41:14	ID requested
⏵ 1	2026-06-14 19:31:44	announced

Mearns H, Long JS, Lilla S, Hodge K, Ladds MJGW, Nixon C, Fern, á, ndez-Palanca P, Zanivan S, Acedo P, Pereira SP, Ryan KM, Identification of serum protein biomarkers for pre-cancerous lesions associated with pancreatic ductal adenocarcinoma. Mol Oncol, 20(6):1473-1493(2026) [pubmed]

10.1002/1878-0261.70213;

submitter keyword: autophagy, early detection, serum, mice, biomarker,pancreatic ductal adenocarcinoma

pancreatic intraepithelial neoplasia

Kevin Ryan
contact affiliation	CRUK -CRUK Scotland nstitute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom
contact email	k.ryan@crukscotlandinstitute.ac.uk
lab head
Sergio Lilla
contact affiliation	Proteomics
contact email	s.lilla@beatson.gla.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065581
     1. Label: PRIDE project
     2. Name: Identification of serum protein biomarkers for pre-cancerous lesions associated with Pancreatic Ductal Adenocarcinoma (Part3:Human)