PXD065565-1

PXD065565 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of paired glioblastoma stem cells and non-stem cells with focus on PIWI proteins
Description	PIWI proteins, a conserved subfamily of Argonaute proteins that interact with PIWI-interacting RNAs (piRNAs), have been implicated in the pathogenesis of several cancers, including glioblastoma (GBM). This project investigates the expression patterns of the four human PIWI proteins in GBM tissues and glioblastoma stem cells (GSCs) – a therapy-resistant cell population that contributes to tumor aggressiveness and recurrence. We conducted a comprehensive analysis of PIWI expression in GBM. Our results identify PIWIL2 as the predominant PIWI protein significantly enriched in GSCs. Moreover, we report the first evidence of PIWIL4 expression in GBM and question the previously assumed roles of PIWIL1 and PIWIL3 in this context. The deposited data represent mass spectrometry analysis of paired GSCs and non-stem cells derived from human GBMs. In addition, it contains data from GSCs differentiated using 10% fetal calf serum for at least 10 days.
HostingRepository	PRIDE
AnnounceDate	2026-08-04
AnnouncementXML	Submission_2026-08-03_21:02:52.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakub Cervenka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-27 08:48:46	ID requested
⏵ 1	2026-08-03 21:02:53	announced

Dataset with its publication pending

submitter keyword: None

Helena Kupcova Skalníkova
contact affiliation	Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic
contact email	helena.skalnikova@lf1.cuni.cz
lab head
Jakub Cervenka
contact affiliation	Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic
contact email	jakub.cervenka@lf1.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD065565

PRIDE project URI

• PRIDE
  1. PXD065565
     1. Label: PRIDE project
     2. Name: Proteomic analysis of paired glioblastoma stem cells and non-stem cells with focus on PIWI proteins