PXD065561-2

PXD065561 is an original dataset announced via ProteomeXchange.

Title	Cooperative clamp-mediated promoter recognition by poxviral RNA polymerase and its TBP/TFIIB-like partner
Description	The recruitment of RNA polymerase (RNAP) to gene promoters is a critical step in gene expression. For RNAP II, this process is initiated by the TATA-box binding protein (TBP) and transcription factor IIB (TFIIB), with homologs of these TBP/TFIIB pairs found in all known multi-subunit RNAP systems. Here we describe a previously unknown mode of promoter recognition by the poxviral intermediate transcription factor 3 (VITF-3). This heterodimeric factor comprises an atypical TBP/TFIIB pair forming a stable ring structure inert towards DNA in the absence of viral RNAP (vRNAP). Promoter recognition instead requires concerted VITF-3 and vRNAP binding, as revealed by cryo-EM analysis of the intermediate pre-initiation complex (iPIC). During iPIC formation, vRNAP facilitates ring opening and loading of VITF-3 onto the promoter, anchoring the polymerase at the transcription start site. Our findings propose vRNAP as a clamp loader for VITF-3 and reveal a thus far unknown transcription initiation mechanism.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-26_03:57:14.996.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Jungwirth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Vaccinia virus; NCBI TaxID: NEWT:10245;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-27 06:59:38	ID requested
1	2025-12-17 04:11:42	announced
⏵ 2	2026-01-26 03:57:15	announced	2026-01-26: Updated project metadata.

Dataset with its publication pending

submitter keyword: Vaccinia virus, clamp-loading, TBP/TFIIB-like, cryo-electron microscopy, Pre-initiation complex, non-canonical promoter recognition, transcription initiation

Utz Fischer
contact affiliation	Department of Biochemistry 1, Theodor Boveri-Institute, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
contact email	utz.fischer@biozentrum.uni-wuerzburg.de
lab head
Stefan Jungwirth
contact affiliation	Julius-Maximilians-Universität Würzburg
contact email	stefan.jungwirth@uni-wuerzburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD065561

PRIDE project URI

• PRIDE
  1. PXD065561
     1. Label: PRIDE project
     2. Name: Cooperative clamp-mediated promoter recognition by poxviral RNA polymerase and its TBP/TFIIB-like partner