⮝ Full datasets listing
PXD065553-1
PXD065553 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Secretion of Hcp1 by Burkholderia pseudomallei Potentiates MNGCs Formation through E-selectin Upregulation |
| Description | Multinucleated giant cells (MNGCs) facilitate pathogen dissemination, yet their formation mechanisms remain poorly understood. This study uncovered a novel pathogenic strategy by which Burkholderia pseudomallei exploited its type VI secretion system (T6SS) effector Hcp1 to drive MNGCs formation. Through integrative transcriptomic and mass spectrometry analyses, we demonstrated that Hcp1 specifically targeted arginine residues 78 and 86 of host Arginase-1 (ARG1), enhancing its enzymatic activity to reprogram arginine metabolism toward polyamine biosynthesis. This metabolic shifted triggered E-selectin transcriptional activation, a prerequisite for cell fusion and MNGCs assembly. Significantly, pharmacological inhibition of ARG1 or genetic ablation of E-selectin confered robust protection against B. pseudomallei infection in murine models, reducing pathogen burden by 65%-76%. Our findings established a mechanistic framework linking bacterial effector-mediated metabolic manipulation to MNGCs formation, while identifying ARG1/E-selectin signaling as a promising host-directed therapeutic axis against intracellular pathogens. This work advances the paradigm of pathogen-host metabolic interplay and provides translatable insights for combating antibiotic-resistant infections. |
| HostingRepository | iProX |
| AnnounceDate | 2025-06-27 |
| AnnouncementXML | Submission_2026-07-26_18:37:29.644.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pan Wu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-27 01:39:47 | ID requested | |
| ⏵ 1 | 2026-07-26 18:37:30 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Burkholderia pseudomallei, Multinucleated giant cells, type VI secretion system, Hcp1 |
Contact List
| Qian Li | |
|---|---|
| contact affiliation | Department of Clinical Microbiology and Immunology, College of Pharmacy and Laboratory Medicine, Army Medical University |
| contact email | liqianjane@tmmu.edu.cn |
| lab head | |
| Pan Wu | |
| contact affiliation | Department of Clinical Microbiology and Immunology, College of Pharmacy and Laboratory Medicine, Army Medical University |
| contact email | panwu183@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
