PXD065523-1

PXD065523 is an original dataset announced via ProteomeXchange.

Title	SMARCA2/4 degradation tails mediate recruitment of two different E3 ligases
Description	In this work, we characterise novel SMARCA2/4 degraders using a robust cell biology and structural workflow and reveal for the first time, the recruitment of two E3 ligase substrate receptors: FBXO22 and DCAF16 by the same degradation tail. Furthermore, we were able to chemically fine-tune this dual ligase dependency dialling out DCAF16 providing insights into the mechanisms for DCAF16 and FBXO22 covalent recruitment. Dual ligase recruitment may serve to overcome E3 ligase acquired resistance that may feature with clinical use of degraders.
HostingRepository	PRIDE
AnnounceDate	2026-05-16
AnnouncementXML	Submission_2026-05-16_02:41:14.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gajanan Sathe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-26 09:43:37	ID requested
⏵ 1	2026-05-16 02:41:15	announced

10.1038/s41589-026-02224-y;

Spiteri VA, Segal D, Correa-S, á, ez A, Iso K, Casement R, Mu, ñ, oz I Ordo, ñ, o M, Nakasone MA, Sathe G, Sch, ä, tz C, Peters HE, Doward M, Kainacher L, Cowan AD, Ciulli A, Winter GE, Dual E3 ligase recruitment by monovalent degraders for tunable SMARCA 2/4 degradation. Nat Chem Biol, ():(2026) [pubmed]

submitter keyword: E3 ligases  , SMARCA2/4, Protein degrader

Alessio Ciulli
contact affiliation	Professor • Centre for Targeted Protein Degradation
contact email	a.ciulli@dundee.ac.uk
lab head
Gajanan Sathe
contact affiliation	Centre for Targeted Protein Degradation (CeTPD) University of Dundee
contact email	gsathe001@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065523

PRIDE project URI

• PRIDE
  1. PXD065523
     1. Label: PRIDE project
     2. Name: SMARCA2/4 degradation tails mediate recruitment of two different E3 ligases