PXD065490-1

PXD065490 is an original dataset announced via ProteomeXchange.

Title	Human Placenta Extracellular Matrix Hydrogels for wound healing
Description	Injectable hydrogels derived from decellularized extracellular matrix (ECM) are emerging biomaterials for minimally invasive tissue repair. Among available sources, human placental tissue is appealing due to its abundance, ethical acceptability, and structural similarity to fetal tissue environments. In this study, we developed and evaluated hydrogels derived from decellularized human placental extracellular matrix (PECM), with and without crosslinking using tannic acid (TA), to enhance their mechanical strength and therapeutic performance. A perfusion-based decellularization protocol, combining enzymatic and detergent treatments, effectively removed cellular material while preserving key matrix components, such as collagen and glycosaminoglycans. The ECM was then freeze-dried, cryo-milled, and enzymatically digested to form hydrogels at concentrations of 10, 15, and 20 mg/mL, which were neutralized and gelled at body temperature (37 °C). To reinforce structure and functionality, the hydrogel was crosslinked with TA, a natural polyphenol known for its biocompatibility and antioxidant effects. TA-crosslinked hydrogels showed significantly improved mechanical properties, including higher storage modulus and slower degradation, while maintaining excellent injectability. Proteomic analysis confirmed the presence of regenerative proteins such as Serpin E1 and IGFBP1, indicating retention of bioactive signaling cues. In vitro, these hydrogels supported healthy growth of endothelial and fibroblast cells, showed good blood compatibility, and exhibited antioxidant and antibacterial activity, particularly in the crosslinked formulations. In vivo experiments in both healthy and diabetic mouse wound models showed that 15 mg/mL PECM hydrogels, especially those with TA, accelerated wound healing, enhanced blood vessel formation, reduced inflammation, and promoted tissue regeneration. Overall, this work offers a reproducible and scalable approach to creating bioactive, mechanically resilient hydrogels. The combination of optimized ECM concentration and TA crosslinking provides a versatile platform for next-generation injectable treatments in regenerative medicine.
HostingRepository	PRIDE
AnnounceDate	2026-01-20
AnnouncementXML	Submission_2026-01-20_01:35:33.377.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065490
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Santosh Mathapati
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 22:56:30	ID requested
⏵ 1	2026-01-20 01:35:34	announced

Gujjar S, Jaipal P, Jayabal P, Panda BN, Sharma P, Sharma JC, Pandey AK, Mathapati S, Bioactive human placental ECM hydrogels crosslinked with tannic acid enhance stability and antioxidant properties for diabetic wound healing. Colloids Surf B Biointerfaces, 260():115406(2026) [pubmed]

10.1016/j.colsurfb.2025.115406;

10.6019/PXD065490;

submitter keyword: Decellularization, Regenerative Medicine,Human Placenta, Extracellular Matrix, Hydrogel

Santosh Mathapati
contact affiliation	Center for Biodesign and Diagnostics
contact email	santosh@thsti.res.in
lab head
Santosh Mathapati
contact affiliation	Translational Health Science and Technology Institute
contact email	santosh@thsti.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065490
     1. Label: PRIDE project
     2. Name: Human Placenta Extracellular Matrix Hydrogels for wound healing