PXD065457-1

PXD065457 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome integral solubility alteration assay for monitoring antibody-antigen interactions in cells
Description	Proteome Integral Solubility Alteration (PISA) assay is normally used for characterization of the interactions between a small molecule and the cellular proteome. Here we modified PISA uniquely to identify the antigen (tumor necrosis factor alpha) in activated monocytes using the monoclonal antibody Infliximab.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_14:22:49.850.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susanna Lundstrom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 11:01:13	ID requested
⏵ 1	2026-05-20 14:22:50	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: PISA, Antibody, Antigen

submitter keyword: PISA, Antibody, Antigen

Contact List

Roman Zubarev
contact affiliation	Division of Chemistry I, MBB, Karolinska Institutet
contact email	Roman.Zubarev@ki.se
lab head
Susanna Lundstrom
contact affiliation	Division of Chemistry I Department of Medical Biochemistry & Biophysics Karolinska Institutet
contact email	susanna.lundstrom@ki.se
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065457
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065457

PRIDE project URI

Repository Record List

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