PXD065451-1

PXD065451 is an original dataset announced via ProteomeXchange.

Title	Chronic Perinatal Oxycodone Exposure Alters Placental EV Proteome and Fetal Cardiomyopathy-Linked Pathways
Description	The rising prevalence of opioid use during pregnancy poses serious public health concerns, yet the molecular mechanisms by which opioids such as oxycodone impact fetal development remain poorly understood. This study investigates the effects of chronic perinatal oxycodone exposure on the composition and signaling functions of placenta-derived extracellular vesicles (EVs) using a rat model. EVs were isolated and characterized via nanoparticle tracking analysis, transmission electron microscopy, Western blotting, and label-free quantitative proteomics. Bioinformatic enrichment analyses revealed that oxycodone exposure significantly altered EV biophysical properties and protein cargo, with pronounced downregulation of cardiomyopathy-associated pathways—including hypertrophic and dilated cardiomyopathy. Key EV proteins such as Atp2a2, Lmna, Tgfb3, Agt, and Sgce, crucial for myocardial function, calcium homeostasis, and vascular signaling, were suppressed, suggesting impaired fetal cardiac programming. Additional pathway analyses indicated disruption of metabolic and glycosylation processes and upregulation of vesicle transport and neurodevelopment-related proteins. These findings position placental EVs as sensitive indicators of in utero drug exposure and propose a mechanistic link between maternal opioid use and altered fetal cardiovascular development. This work establishes a novel systems-level foundation for developing EV-based diagnostics and interventions targeting opioid-induced developmental risk.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:14:10.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gurudutt Pendyala
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 09:21:41	ID requested
⏵ 1	2026-04-26 16:14:11	announced

10.20517/evcna.2025.138;

Foroughi-Nezhad A, Moore D, Schaal VL, Hediyal TA, Stone E, Kolli S, Athota P, Shukri O, Yelamanchili SV, Pendyala G, Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways. Extracell Vesicles Circ Nucl Acids, 7(1):146-164(2026) [pubmed]

submitter keyword: EV biomarkers,Oxycodone, Perinatal exposure, Cardiomyopathy, Placental signaling, Proteomics, Fetal development, Extracellular vesicles (EVs)

Dr. Gurudutt Pendyala
contact affiliation	Robert Lieberman Endowed Professor of Anesthesiology University of Nebraska Medical Center College of Medicine, Department of Anesthesiology
contact email	gpendyala@unmc.edu
lab head
Gurudutt Pendyala
contact affiliation	University of Nebraska Medical Center
contact email	gpendyala@unmc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065451
     1. Label: PRIDE project
     2. Name: Chronic Perinatal Oxycodone Exposure Alters Placental EV Proteome and Fetal Cardiomyopathy-Linked Pathways