PXD065356 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LENG8 mediates nuclear retention and degradation of misprocessed mRNAs and noncoding RNAs |
| Description | Incompletely processed and misprocessed mRNAs as well as numerous noncoding RNAs are retained in the nucleus and often degraded, and defects in this quality control mechanism have been linked to various diseases, including cancer and neurodegeneration. However, the underlying mechanisms remain poorly understood. Here we show that LENG8 is recruited to pre-mRNAs by splicing factors, including the U1 snRNP. Once RNA-bound, LENG8 is both necessary and sufficient to sequester RNA in the nucleus. LENG8 depletion leads to the leakage of misprocessed mRNAs, including intronically polyadenylated and intron-retained mRNAs, as well as noncoding RNAs into the cytoplasm. Mechanistically, we showed that LENG8 binds to PCID2 and SEM1 to form an evolutionarily conserved complex, referred to as REX (repressor of export), and causes RNA nuclear retention by acting as a dominant negative factor for the essential mRNA export factor TREX2. Finally, LENG8 promotes RNA degradation by recruiting the ZFC3H1-MTR4-exosome complex. Thus, LENG8 is a key quality control factor that keeps pre-mRNAs in the nucleus until they are fully and correctly processed. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-30 |
| AnnouncementXML | Submission_2026-03-29_16:35:17.236.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Clinton Yu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-23 16:36:01 | ID requested | |
| ⏵ 1 | 2026-03-29 16:35:18 | announced | |
Publication List
| Tian L, Liu L, Yoon Y, Soles LV, Valdez M, Jeong J, Yu C, Huang L, Shi Y, LENG8 mediates RNA nuclear retention and degradation in eukaryotes. Mol Cell, ():(2026) [pubmed] |
| 10.1016/j.molcel.2026.02.023; |
Keyword List
| submitter keyword: Misprocessed RNAs, RNA nuclear retention, RNA degradation, LENG8 |
Contact List
| Yongsheng Shi |
|---|
| contact affiliation | Department of Microbiology & Molecular Genetics University of California, Irvine Irvine, California, 92697 USA |
| contact email | yongshes@uci.edu |
| lab head | |
| Clinton Yu |
|---|
| contact affiliation | University of California, Irvine |
| contact email | clinton.yu@uci.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD065356 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065356
- Label: PRIDE project
- Name: LENG8 mediates nuclear retention and degradation of misprocessed mRNAs and noncoding RNAs
