PXD065346-1

PXD065346 is an original dataset announced via ProteomeXchange.

Title	Evaluation of proteomics workflows for the quantification of (small) proteins
Description	Quantitative information on protein abundance is crucial to understand biological processes and is therefore frequently gathered in proteomic studies. However, the quality of a quantitative proteomic dataset is greatly affected by the number of missing values, which need to be minimized to produce robust and meaningful data. In this context, small proteins (≤100 amino acids) pose specific analytical challenges which hinder their efficient identification and quantitative characterization in complex proteomes. In this study, methods for sample preparation and MS-data processing are systematically evaluated for their contribution to identification and quantification of small proteins of C. difficile 630 Δerm.
HostingRepository	PRIDE
AnnounceDate	2026-01-19
AnnouncementXML	Submission_2026-01-19_10:10:47.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: NEWT:272563;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 09:15:44	ID requested
⏵ 1	2026-01-19 10:10:47	announced

10.1093/FEMSML/UQAG002;

submitter keyword: Evaluation of proteomics workflows for the quantification of (small) proteins

Dörte Becher
contact affiliation	University of Greifswald, Institute of Microbiology, Department of Microbial Proteomics, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065346
     1. Label: PRIDE project
     2. Name: Evaluation of proteomics workflows for the quantification of (small) proteins