PXD065329-1

PXD065329 is an original dataset announced via ProteomeXchange.

Title	OH2 oncolytic virus inhibits non-small-cell lung cancer metastasis via β-catenin pathway suppression
Description	The five-year survival rate for non-small-cell lung cancer (NSCLC) remains poor, primarily due to tumor invasion and metastasis. This study evaluates the anti-metastatic potential of the oncolytic virus OH2 in NSCLC. OH2 inhibits migration and invasion of NSCLC by downregulating β-catenin, as demonstrated in vitro and an in a lung metastasis model. OH2 reduces β-catenin mRNA levels, suppressing its transcriptional activity and downstream expression of Matrix Metalloproteinases (MMPs), key mediators of extracellular matrix degradation. Proteomic analysis of the secretome confirms reduced MMPs expression following OH2 treatment. Mechanistically, the OH2 tegument protein UL41 is identified as a critical factor that degrades β-catenin mRNA, thus inhibiting β-catenin nuclear transcriptional activity. These findings reveal a novel anti-metastatic mechanism of OH2 via disruption of the β-catenin/MMPs axis and support its potential as a therapeutic candidate for invasive NSCLC.
HostingRepository	PRIDE
AnnounceDate	2025-07-27
AnnouncementXML	Submission_2025-07-26_20:29:48.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Han Hu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 06:21:49	ID requested
⏵ 1	2025-07-26 20:29:48	announced

10.1038/S42003-025-08520-Y;

submitter keyword: Oncolytic Virotherapy

Metastasis Inhibition

Herpes simplex virus

Beta-Catenin

Non-Small Cell Lung Cancer

Han Hu
contact affiliation	Hubei University of Technology
contact email	huh@hbut.edu.cn
lab head
Han Hu
contact affiliation	Hubei University of Technology
contact email	huh@hbut.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065329
     1. Label: PRIDE project
     2. Name: OH2 oncolytic virus inhibits non-small-cell lung cancer metastasis via β-catenin pathway suppression