PXD065318-1

PXD065318 is an original dataset announced via ProteomeXchange.

Title	Impact of SliP4 deletion on the high-light acclimation in Synechocystis sp. PCC 6803
Description	SliP4 is a 37 amino acids small protein that is strongly induced when the cyanobacterium Synechocystis is exposed to high-light conditions. Deletion mutants exhibit a light-sensitive phenotype due to impaired cyclic electron flow and state transitions. Here, we investigated the consequences of SliP4 deficiency on the high-light acclimation. The ∆sliP4 mutant exhibited a fully intact gene regulatory response 30 min after shifting the light intensity from 50 to 250 μmol photons m-2 s-1 mediated by the RpaB-PsrR1 system. However, 48 h after the shift to high light, the mutant increased the production of extracellular polysaccharides and the cells started to aggregate, effectively reducing the potential impact of light stress effects caused by the impaired capacity for cyclic electron flow and state transitions. This effect included the upregulation of xssA-E and xssN-P genes for the production of synechan, a sulfated exopolysaccharide. Our results show that the unicellular cyanobacterium Synechocystis can compensate the crucial role of SliP4 by the activation of a population-level response to cope with light stress conditions and reveal several genes involved in this response.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:43:42.907.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: NEWT:1148;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 02:59:47	ID requested
⏵ 1	2026-02-08 16:43:45	announced

10.1093/femsml/uqag003;

Alvarenga-Lucius L, Maa, ß S, Reimann V, Becher D, Hess WR, Hagemann M, sp. PCC 6803. Microlife, 7():uqag003(2026) [pubmed]

submitter keyword: metabolome, high light, photosynthesis,Gene expression, proteome

Dörte Becher
contact affiliation	Institute of Microbiology, Department of Microbial Proteomics, University of Greifswald, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065318
     1. Label: PRIDE project
     2. Name: Impact of SliP4 deletion on the high-light acclimation in Synechocystis sp. PCC 6803