PXD065309-1

PXD065309 is an original dataset announced via ProteomeXchange.

Title	Blockade of mitochondrial components release by exosome pathway promotes the pathogenesis of Fuchs endothelial corneal dystrophy
Description	Fuchs endothelial corneal dystrophy (FECD) is the leading indication of corneal transplantation worldwide and the focus of pathogenesis has been on the corneal endothelium. Instead of cellular analysis, we aimed to identify the protein changes of aqueous humor (AH) in patients with FECD and investigate in more detail the relationship between AH and corneal endothelium. We collected 13 AH samples of 7 early/middle stage FECD patients and 6 control patients during routine cataract surgery. The proteomes of AH were profiled with the 4D label-free quantitative tandem mass spectrometry. Among 1613 identified proteins, 44 proteins exhibited above two-fold upregulation in the AH of FECD patients than control patients. Gene ontology (GO) analysis showed the enrichment of mitochondrial components, which were further validated by ELISA of mitochondrial proteins SLC25A3, PC, and PARK7. Moreover, immunofluorescence staining and ultrastructural observation were conducted in clinical specimens, mouse corneal endothelium and cultured human corneal endothelial cells (HCECs). The mitochondrial protein TOM20 was reduced in the FECD corneal endothelium, accompanied by damaged mitochondrial ejection. We next isolated extracellular vesicles by ultracentrifugation from HCECs and revealed that the mitochondria copy numbers were significantly increased in UVA-irradiated cells. Inhibition of exosome biogenesis aggravated cell death and mitochondrial membrane potential impairment in FECD endothelial cells. Taken together, our results provided novel insights into the proteome characterization of the AH from FECD patients and offered new perspective to deepen the impaired mitochondrial quality control in the pathogenesis of FECD.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_01:43:47.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Can Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 00:20:03	ID requested
⏵ 1	2026-01-23 01:43:48	announced

10.1038/s41420-025-02881-3;

Zhao C, Wang Q, Zhou Q, Wang Z, Yao S, Sang T, Duan H, Wu J, Zhong X, Sui X, Shi W, Wang T, Blockade of mitochondrial components release by exosome pathway promotes the pathogenesis of Fuchs endothelial corneal dystrophy. Cell Death Discov, 12(1):30(2025) [pubmed]

submitter keyword: Fuchs endothelial corneal dystrophy

proteomics

aqueous humor

corneal endothelium

mitochondrial quality control

Can Zhao
contact affiliation	Eye Institute of Shandong First Medical University，Eye Hospital of Shandong First Medical University (Shandong Eye Hospital), Jinan, China
contact email	zhaocan0914@163.com
lab head
Can Zhao
contact affiliation	Eye Hospital of Shandong First Medical University, Shandong Eye Hospital
contact email	zhaocan0914@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065309
     1. Label: PRIDE project
     2. Name: Blockade of mitochondrial components release by exosome pathway promotes the pathogenesis of Fuchs endothelial corneal dystrophy