PXD065294-1

PXD065294 is an original dataset announced via ProteomeXchange.

Title	PRMT5 activity sustains histone production to maintain genome integrity
Description	Histone proteins package DNA into nucleosomes, forming chromatin and thereby safeguarding genome integrity. Proper histone expression is essential for cell proliferation and chromatin organization, yet the upstream regulators of histone supply remain in-completely understood. PRMT5—a cell essential type II protein arginine methyltransferase frequently overexpressed in cancer—catalyzes symmetric dimethylation of arginine residues and is known to regulate transcription, splicing, and mRNA sub-cellular transit. Despite the pre-clinical development of PRMT5 inhibitors, its crucial molecular functions in proliferating cells remain unre-solved. Here, we combine temporal chemical inhibition, multi-omic profiling, and high-resolution imaging to define the immediate cellular consequences of PRMT5 loss. We find that PRMT5 activity is required to sustain histone gene transcription and histone protein production during S phase, and that its inhibition leads to rapid reduction of histone mRNA, reduced histone protein levels, and replication-associated stress, as indicated by micronuclei formation and cellular DNA loss. These findings establish PRMT5 as a critical regulator of histone homeostasis and reveal a previously unrecognized mechanism linking arginine methylation to chromatin integrity and genome stability.
HostingRepository	PRIDE
AnnounceDate	2026-09-08
AnnouncementXML	Submission_2026-09-08_12:49:11.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Sidoli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-22 13:53:32	ID requested
⏵ 1	2026-09-08 12:49:12	announced

Dataset with its publication pending

submitter keyword: Histone Transcription, PRMT5,Arginine Methylation, Arginine Methyltransferase, Genome Integ-rity, Post-Translational Modifications

Simone Sidoli
contact affiliation	Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States
contact email	simone.sidoli@einsteinmed.edu
lab head
Simone Sidoli
contact affiliation	Albert Einstein College of Medicine
contact email	simone.sidoli@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD065294

PRIDE project URI

• PRIDE
  1. PXD065294
     1. Label: PRIDE project
     2. Name: PRMT5 activity sustains histone production to maintain genome integrity