⮝ Full datasets listing
PXD065275-1
PXD065275 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A conserved role for the Zinc binding ZZ domain of the ORB2 CPEB2 RNA binding protein in translational repression of target transcripts in Drosophila S2 cells and embryos |
| Description | RNA binding proteins RBPs are key components of the post transcriptional regulatory machinery. We show that the ORB2 RBP, the Drosophila ortholog of vertebrate Cytoplasmic Polyadenylation Element Binding Protein 2 CPEB2, binds to hundreds of maternally provided mRNAs in early embryos, these transcripts are translationally repressed and unstable during the maternal to zygotic transition MZT. We identify a U rich motif enriched in ORB2 targets, which confers ORB2 binding and repression to a luciferase reporter mRNA in S2 tissue culture cells. ORB2 and its human homolog CPEB2 but not ORB and CPEB1 repress translation. The C terminal Zinc binding ZZ domain of ORB2 is necessary and sufficient for repression. We mapped regions of ORB2 ZZ domain that, when substituted into the same region of ORB ZZ domain, converts the non repressive domain into a repressor. ORB2 interacts with a suite of post-transcriptional regulators in early embryos; a subset of these interactions is lost upon deletion of the ZZ domain, notably with the Cup repressive complex. Analysis of the early embryo translatome in the presence or absence of the endogenous ZZ domain, shows that ORB2 targets move onto polysomes upon ZZ domain deletion, indicating that this domain mediates translational repression of ORB2 targets during the MZT. Together, our results assign a function to the ZZ domain and support a significant role for ORB2 in post transcriptional regulation of maternal mRNAs during the Drosophila MZT. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-04 |
| AnnouncementXML | Submission_2025-11-04_17:08:25.638.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sichun Lin |
| SpeciesList | scientific name: Drosophila |
| ModificationList | Oxidation |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-20 10:06:16 | ID requested | |
| ⏵ 1 | 2025-11-04 17:08:26 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: ORB2, Cytoplasmic Polyadenylation Element Binding Protein CPEB, RNA binding protein, translational regulation, post transcriptional regulation, Drosophila, embryo, maternal to zygotic transition, S2 cell, CPEB, Cup, Trailer hitch TRAL, ME31B, Belle BEL, Smaug SMG, DatasetType:Proteomics |
Contact List
| Howard Lipshitz | |
|---|---|
| contact affiliation | University of Toronto |
| contact email | howard.lipshitz@utoronto.ca |
| lab head | |
| Sichun Lin | |
| contact affiliation | University of Toronto, Leslie Dan Faculty of Pharm |
| contact email | sichun.lin@utoronto.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098275/ |
