PXD065251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SILAC IP-Mass spectrometry of TTLL-transfected HEK cells |
| Description | Identification of the non-tubulin substrates of tubulin glycylases, TTLL3/TTLL8 and tubulin glutamylases TTLL4/TTLL5 using SILAC-IP-MS/MS quantitative mass spectrometry approach. HEK cells transfected with the respective enzymes were lysed and the proteins modified by the enzymes enriched by immunopulldown using modification specific antibodies and subsequently, the potential substrates identified using quantitative mass spectrometry. The identity of the proteins was further validated by cellular and in vitro modification assays |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-18 |
| AnnouncementXML | Submission_2026-05-18_08:33:18.281.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Vanessa Masson |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
| 0 | 2025-06-20 01:22:52 | ID requested | |
| ⏵ 1 | 2026-05-18 08:33:18 | announced | |
Publication List
Keyword List
| submitter keyword: HEK cells, TTLLs, IP-MS/MS,SILAC |
Contact List
| Damarys Loew |
| contact affiliation | Head of the Curie Institute Mass Spectrometry Platform |
| contact email | damarys.loew@curie.fr |
| lab head | |
| Vanessa Masson |
| contact affiliation | Institut Curie - Centre de Recherche |
| contact email | vanessa.masson@curie.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065251 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065251
- Label: PRIDE project
- Name: SILAC IP-Mass spectrometry of TTLL-transfected HEK cells