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PXD065251-1

PXD065251 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSILAC IP-Mass spectrometry of TTLL-transfected HEK cells
DescriptionIdentification of the non-tubulin substrates of tubulin glycylases, TTLL3/TTLL8 and tubulin glutamylases TTLL4/TTLL5 using SILAC-IP-MS/MS quantitative mass spectrometry approach. HEK cells transfected with the respective enzymes were lysed and the proteins modified by the enzymes enriched by immunopulldown using modification specific antibodies and subsequently, the potential substrates identified using quantitative mass spectrometry. The identity of the proteins was further validated by cellular and in vitro modification assays
HostingRepositoryPRIDE
AnnounceDate2026-05-18
AnnouncementXMLSubmission_2026-05-18_08:33:18.281.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVanessa Masson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-06-20 01:22:52ID requested
12026-05-18 08:33:18announced
Publication List
10.1242/JCS.264734;
Keyword List
submitter keyword: HEK cells, TTLLs, IP-MS/MS,SILAC
Contact List
Damarys Loew
contact affiliationHead of the Curie Institute Mass Spectrometry Platform
contact emaildamarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliationInstitut Curie - Centre de Recherche
contact emailvanessa.masson@curie.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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