PXD065220-1

PXD065220 is an original dataset announced via ProteomeXchange.

Title	A Rab1 interactome illuminates its dual role in autophagy and membrane trafficking
Description	The small GTPase Rab1 is found in all eukaryotes and acts in both ER-to-Golgi transport and autophagy. Several Rab1 effectors and regulators have been identified, but the mechanism by which Rab1 orchestrates these distinct processes remains incompletely understood. We apply MitoID, a proximity biotinylation approach, to expand the interactome of human Rab1A and Rab1B. We identify new interactors amongst known membrane traffic and autophagy machinery, as well as previously uncharacterised proteins. One striking set of interactors are the cargo receptors for selective autophagy, indicating a broader role for Rab1 in autophagy than previously supposed. Two cargo receptor interactions are validated in vitro, with the Rab1 binding site in the cargo receptor optineurin being required for mitophagy in vivo. We also find an interaction between Rab1 and the dynein adaptor FHIP2A that can only be detected in the presence of membranes. This explains recruitment of dynein to the ER-Golgi-intermediate compartment and demonstrates that conventional methods can miss a subset of effectors of small GTPases.
HostingRepository	PRIDE
AnnounceDate	2025-12-18
AnnouncementXML	Submission_2025-12-18_01:48:34.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina Franco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-19 07:56:50	ID requested
⏵ 1	2025-12-18 01:48:34	announced

Dataset with its publication pending

submitter keyword: membrane trafficking, autophagy,Rab1 , interactome

Sean Munro
contact affiliation	Division of Cell Biology MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH, UK
contact email	sean@mrc-lmb.cam.ac.uk
lab head
Catarina Franco
contact affiliation	MRC Laboratory of Molecular Biology
contact email	cffranco@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065220
     1. Label: PRIDE project
     2. Name: A Rab1 interactome illuminates its dual role in autophagy and membrane trafficking