PXD065218-1

PXD065218 is an original dataset announced via ProteomeXchange.

Title	The functional antagonism between SIRT2 and MOF regulates mitotic entry - PLK1 methylation
Description	Mitosis entry is tightly regulated by a complex network of interconnected mechanisms involving epigenetic modifications, signaling pathways, transcriptional control, and structural changes. While significant progress has been made in understanding these processes individually, the mechanisms integrating chromatin dynamics with key mitotic regulators are still not fully understood. Here, we identify a functional antagonism between the deacetylase SIRT2 and the acetyltransferase MOF in the G2/M transition and mitotic progression. This interplay, which involves MOF deacetylation by SIRT2, regulates key histone marks, including H4K16ac deacetylation and H4K20me1 deposition, condensin II loading, the stability of the key mitotic regulator PLK1 and has a major impact on the transcription control of mitosis regulated by the FOXM1. Our findings reveal a previously unrecognized layer of regulation in G2/M progression, shedding light on the intricate crosstalk between chromatin dynamics and mitotic control, with potential implications for understanding chromosomal stability and cancer.
HostingRepository	PRIDE
AnnounceDate	2026-04-29
AnnouncementXML	Submission_2026-04-29_00:01:35.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joan Josep Bech-Serra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monomethylated residue; deamidated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-19 06:22:59	ID requested
⏵ 1	2026-04-29 00:01:36	announced

Dataset with its publication pending

submitter keyword: PLK1, MOF, Orbitrap eclipse,SIRT2, label free

Alex Vaquero
contact affiliation	Chromatin Biology Laboratory, Cancer Epigenetics and Biology Program (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain. Chromatin Biology Laboratory, Josep Carreras Leukaemia Research Institute (IJC), Badalona, Spain.
contact email	avaquero@carrerasresearch.org
lab head
Joan Josep Bech-Serra
contact affiliation	Proteomics Unit - Josep Carreras Leukaemia Research Institute
contact email	jbech@carrerasresearch.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065218
     1. Label: PRIDE project
     2. Name: The functional antagonism between SIRT2 and MOF regulates mitotic entry - PLK1 methylation