PXD065186-1

PXD065186 is an original dataset announced via ProteomeXchange.

Title	Homologous HipA-like kinases are controlled by internal translational initiation and genetic organisation
Description	HipA-like kinases are widespread bacterial serine-threonine kinases, yet their regulatory mechanisms remain poorly understood. Here, we characterise two novel HipA-like systems, the monocistronic hipL and bicistronic hipIN, containing a HipS-like and a HIRAN domain. We show that hipL contains an internal translation initiation site producing a smaller variant, HipLS, which counteracts HipL-mediated toxicity via its HipS-like domain. Contrary to this, HipN requires both the HipS-like and the HIRAN domains to neutralize HipI-mediated toxicity. Neither system forms stable toxin-antitoxin complexes in vitro, distinguishing them from classical type II systems. Finally, we show that autophosphorylation is essential for HipL but not HipI toxicity. These findings reveal diverse regulatory architectures in HipA-like TA systems, shaped by domain composition and operon structure.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-15_08:58:16.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Payal Nashier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-18 18:00:03	ID requested
⏵ 1	2025-12-15 08:58:17	announced

Chrenkov, á A, Nashier P, Madsen CL, Singh M, Nielsen J, Otzen DE, Enghild JJ, Macek B, Skjerning RB, Brodersen DE, Homologous HipA-like kinases are controlled by internal translational initiation and genetic organisation. FEBS Lett, ():(2025) [pubmed]

10.1002/1873-3468.70234;

submitter keyword: tRNA synthetase, E. coli,toxin-antitoxin, HipBST, protein kinase, HipBA

Boris Macek
contact affiliation	Proteome Center Tuebingen, Institute for Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076, Tuebingen, Germany
contact email	boris.macek@uni-tuebingen.de
lab head
Payal Nashier
contact affiliation	Proteome Center Tuebingen, Institute of Cell Biology, University of Tuebingen
contact email	payal.nashier@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065186
     1. Label: PRIDE project
     2. Name: Homologous HipA-like kinases are controlled by internal translational initiation and genetic organisation