PXD065161 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Discovery and Validation of Serum Biomarkers and Neoantigens for Differentiating Colorectal Cancer from Inflammatory Bowel Diseases |
| Description | Early detection of colorectal cancer (CRC) and distinguishing inflammatory bowel diseases from cancerous conditions significantly improve survival rates. While colonoscopy is the gold standard for CRC detection, it is invasive and associated with potential complications. Therefore, there is a need for blood biomarkers that possess high sensitivity and specificity which can be used for pre-diagnosis. In this study, we screened for serum biomarkers specifically altered in colonoscopy-positive (CP) patients (cancer group) when compared to colonoscopy-negative (CN) patients (suffering from other bowel-related illnesses with symptoms similar to CRC), and healthy control (HC) individuals (n=20 each). Using Data-Independent Acquisition-Mass spectrometry (DIA-MS) analysis, we identified potential protein markers and neoantigens uniquely altered in the CP group compared to the CN group. These biomarkers were validated using a targeted approach, Parallel Reaction Monitoring-Mass Spectrometry (PRM-MS). An independent set of samples (n=10 each) was taken for all the groups. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-01 |
| AnnouncementXML | Submission_2026-08-01_00:07:56.891.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | TS Keshava Prasad |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-18 07:56:20 | ID requested | |
| 1 | 2025-06-23 02:16:12 | announced | |
| 2 | 2026-05-01 22:15:31 | announced | 2026-05-02: Updated project metadata. |
| ⏵ 3 | 2026-08-01 00:07:57 | announced | 2026-08-01: Updated project metadata. |
Publication List
| 10.1021/acs.jproteome.5c00285; |
| Rai AB, Codi JAK, Kotimoole CN, Ramesh P, Narayana VK, Shaji V, Prasad TSK, Data-Independent Acquisition Analysis to Identify Serum Biomarkers and Neoantigens for Differentiating Colorectal Cancer from Inflammatory Bowel Diseases. J Proteome Res, 24(8):4166-4180(2025) [pubmed] |
Keyword List
| submitter keyword: colonoscopy, serum biomarkers, pre-diagnosis,cancer, neoantigen |
Contact List
| Dr. T |
|---|
| contact affiliation | Professor and Deputy Director Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, India |
| contact email | tskprasad@gmail.com |
| lab head | |
| TS Keshava Prasad |
|---|
| contact affiliation | Director, Center for Omics & Systems Medicine (C-OSM)
Professor, Department of Biochemistry
K.S. Hegde Medical Academy (KSHEMA)
Nitte (Deemed to be University) |
| contact email | tskprasad@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD065161 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065161
- Label: PRIDE project
- Name: Discovery and Validation of Serum Biomarkers and Neoantigens for Differentiating Colorectal Cancer from Inflammatory Bowel Diseases
