PXD065158 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome-wide identification of ISG15 sites in HeLa cells |
| Description | We employed a GlyGly-peptidomics approach to comprehensively map ISG15 modification sites in HeLa cells under three conditions: mock-transfected controls, IFN-induced cells, and cells transfected with the ISGylation machinery. Lysates from wild-type and Isg15 knockout (KO) cells were processed using S-trap, followed by trypsin digestion to generate diglycine-modified peptides. These peptides were enriched via immunoprecipitation and analyzed by LC-MS/MS for identification and quantification. Comparative analysis across conditions and genotypes revealed 671 ISG15 modification sites on 456 distinct proteins, expanding the HeLa ISGylome. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-02 |
| AnnouncementXML | Submission_2026-03-02_11:50:40.519.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Denzel Eggermont |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-18 05:55:26 | ID requested | |
| ⏵ 1 | 2026-03-02 11:50:41 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: PTM, HeLa, GlyGly,ISG15, GG-peptidomics |
Contact List
| Francis Impens |
|---|
| contact affiliation | VIB-UGent Center for Medical Biotechnology, VIB, Ghent,Belgium |
| contact email | francis.impens@vib-ugent.be |
| lab head | |
| Denzel Eggermont |
|---|
| contact affiliation | VIB-Ugent Center for Medical Biotechnology |
| contact email | denzel.eggermont@vib-ugent.be |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD065158 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065158
- Label: PRIDE project
- Name: Proteome-wide identification of ISG15 sites in HeLa cells
