PXD065135 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The EGF receptor kinase domain is strongly activated by weak dimerization |
| Description | The tyrosine kinase domain (TKD) of the epidermal growth factor receptor (EGFR) activates via a unique mechanism of asymmetric dimerization with a partner EGFR TKD rather than a more typical mechanism of activation loop phosphorylation. There is an EGFR oncogenic variant found in patients in which the TKD region of EGFR has been duplicated, resulting in a construct in which two EGFR kinase domain are linked via a natural peptide linker (KDD). KDD is the best approximation of the natural process of EGFR kinase dimerization and activation as well as an example of bona fide oncogenic activation. We studied the structure, enzyme properties, and structural dynamics of KDD to gather insights into the EGFR kinase activation process and its oncogenicity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-25_06:15:39.115.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zaritza Petrova |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-17 19:40:15 | ID requested | |
| ⏵ 1 | 2025-11-25 06:15:39 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: dynamics, KDD, dimerization, kinase, HDX-MS,EGFR |
Contact List
| Mark Lemmon |
|---|
| contact affiliation | Yale University, Yale Cancer Biology Institute |
| contact email | mark.lemmon@yale.edu |
| lab head | |
| Zaritza Petrova |
|---|
| contact affiliation | Yale University |
| contact email | zaritza.petrova@yale.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD065135 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065135
- Label: PRIDE project
- Name: The EGF receptor kinase domain is strongly activated by weak dimerization
