PXD065129-1

PXD065129 is an original dataset announced via ProteomeXchange.

Title	MAHRP2 is required for tether formation and PfEMP1 trafficking in Plasmodium falciparum infected red blood cells.
Description	The ability of the human malaria parasite Plasmodium falciparum to remodel its host red blood cell (RBC) is central to the parasite’s ability to survive within the circulation of its host and cause disease. Following invasion, the parasite exports proteins into the RBC cytoplasm where they remodel the membrane skeleton and alter the cells biophysical properties. Many of the exported proteins are trafficked through parasite derived structures called Maurer’s clefts in the cytoplasm of the RBC. These clefts act as an intermediate compartment for proteins en route to the RBC membrane such as the major virulence protein, Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1). From the Maurer’s clefts, PfEMP1 is delivered to the RBC membrane through a poorly understood process, which is thought to combine physical tethering of the Maurer’s clefts to the membrane and vesicle mediated trafficking. A tube-like structure called the tether can be seen by electron-tomography connecting the Maurer’s clefts to the RBC membrane. To date, the function and formation of these tether structures are unknown, with only one protein, the Membrane Associated Histidine Rich Protein 2 (MAHRP2) known to locate at these structures. In this study, we show that deletion of MAHRP2 ablates tether formation, affects Maurer’s cleft immobilisation at the RBC membrane, resulting in a significant reduction in PfEMP1 trafficking to the membrane and the parasite’s ability to cytoadhere. Lastly, we show that deletion of MAHRP2 leads to global impacts in PTP2 related vesicle formation and a reduction in J-dot chaperone components.
HostingRepository	PRIDE
AnnounceDate	2026-06-26
AnnouncementXML	Submission_2026-06-25_18:52:21.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 17:15:08	ID requested
⏵ 1	2026-06-25 18:52:22	announced

Shibu MA, Shami GJ, Hanssen E, Carmo OMS, Vaibhav V, Yousef J, Dagley LF, McCarthy J, Dixon MWA, infected red blood cells. Front Cell Infect Microbiol, 16():1675134(2026) [pubmed]

10.3389/fcimb.2026.1675134;

submitter keyword: PfEMP1,MAHRP2, tether, exported proteins, Maurer’s clefts

Matthew W A Dixon
contact affiliation	Walter and Eliza Hall Institute of Medical Research and The University of Melbourne
contact email	matthew.dixon@unimelb.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065129
     1. Label: PRIDE project
     2. Name: MAHRP2 is required for tether formation and PfEMP1 trafficking in Plasmodium falciparum infected red blood cells.