PXD065120-1

PXD065120 is an original dataset announced via ProteomeXchange.

Title	NagA mediates the recycling of peptidoglycan derived amino sugars in mycobacteria
Description	Many bacterial species are known to recover peptidoglycan (PG) fragments released from remodelling of their cell walls during growth and cell division. These PG fragments not only provide an essential energy resource, especially in nutrient restricted environments, but also play a critical role in influencing infection. Yet whether mycobacteria have the capacity to recycle their PG, or not, has yet to be resolved. In this study we show that NagA, an N-acetylglucosamine-6-phosphate (GlcNAc-6-P) deacetylase, is essential for coordinating the recycling of an amino sugar component released from the mycobacterial cell wall. We show that NagA is exclusively responsible for GlcNAc-6-P deacetylation and is pivotal for the de novo synthesis of core cell wall building blocks. Indeed, a nagA mutant exhibited impaired cell wall, defective biofilm formation, and enhanced susceptibility to PG targeting agents. Moreover, uptake analysis and profiling of the amino-sugar pool revealed that NagA inactivation blocks N-acetylglucosamine (GlcNAc) import and has a pronounced effect on the fate and levels of the intracellular amino sugar pool. Loss of NagA led to the up- and down-regulation of proteins involved in cell wall biosynthesis, thereby altering cell wall homeostasis. Overall, our data highlights the importance of an overlooked yet conserved component in an important PG salvage pathway in mycobacteria, in which NagA provides a unique GlcNAc sensing mechanism, thus acting as a checkpoint for regulating the recovery and reuse of PG fragments.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-23_16:33:35.101.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065120
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Mycobacterium smegmatis; NCBI TaxID: NEWT:1772;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 10:07:00	ID requested
⏵ 1	2025-11-23 16:33:36	announced

10.6019/PXD065120;

10.1016/j.jbc.2025.110597;

Guy CS, Cooper C, Karlikowska M, Harrison J, Singh A, Serv, í, n-Gonz, á, lez LS, Evans CA, Bakker SE, Bottrill A, Bhatt A, Mesnage S, Besra GS, Fullam E, The deacetylase NagA mediates the remodeling and recycling of peptidoglycan-derived amino sugars in mycobacteria. J Biol Chem, 301(11):110597(2025) [pubmed]

submitter keyword: Warwick_RTP, peptidoglycan,NagA, mycobacteria

Professor Elizabeth Fullam
contact affiliation	School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK
contact email	elizabeth.fullam@manchester.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065120
     1. Label: PRIDE project
     2. Name: NagA mediates the recycling of peptidoglycan derived amino sugars in mycobacteria