PXD065118-1

PXD065118 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics of rat knee-joint tissue in a monosodium-iodoacetate osteoarthritis model treated with the Ayurvedic formulations
Description	Osteoarthritis (OA) causes progressive cartilage and bone degradation for which no disease-modifying therapy exists. Withania somnifera (WS) and Yogaraj Guggulu (YG) are centuries-old herbal formulation used in Ayurveda for joint disorders, but its molecular mode of action is poorly defined. We performed label-free LC-MS/MS proteomics on rat knee-joint tissue to uncover pathways modulated by oral WS and YG in an MIA-induced OA model. Eighty male Sprague-Dawley rats were randomised into normal control, vehicle, disease control, diclofenac (standard), three YG dose groups (low, medium, high) and three WS groups (low, medium, high). WS or YG or vehicle was given orally for 21 days after OA induction (5 mg MIA, intra-articular). Knee joints were harvested on Day 28, flash-frozen, pulverised and processed for LC-MS/MS. Each biological replicate was injected with three times, resulting in 12 replicates per group. WS and YG normalised multiple OA-dysregulated proteins, dampening NF-κB/IL-17 signalling and collagen-degradation cascades while restoring metabolic and cytoskeletal processes, congruent with micro-CT, histology and behavioural recovery.
HostingRepository	PRIDE
AnnounceDate	2026-06-19
AnnouncementXML	Submission_2026-06-19_14:49:50.785.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD065118
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Amay Redkar
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	iodoacetic acid derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 07:49:15	ID requested
⏵ 1	2026-06-19 14:49:51	announced

10.1016/J.JEP.2026.122058;

10.6019/PXD065118;

submitter keyword: Yogaraj Guggulu, label-free proteomics, Orbitrap Eclipse,Rattus norvegicus, osteoarthritis

Withania somnifera, knee joint, data-dependent acquisition, LC-MS/MS

Vibin Ramakrishnan
contact affiliation	Professor, Biosciences & Bioengineering. Associate Faculty: MF School of Data Science and Artificial Intelligence Indian Institute of Technology Guwahati, Assam, India
contact email	vibin@iitg.ac.in
lab head
Amay Redkar
contact affiliation	Indian Institute of Technology Guwahati
contact email	amay@iitg.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065118
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics of rat knee-joint tissue in a monosodium-iodoacetate osteoarthritis model treated with the Ayurvedic formulations