PXD065061 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ultradynamic protein assemblies and network channeling |
| Description | Whereas a classical view sustains that sequential metabolic reactions and other complex step-wise processes can be efficiently executed by proteins dispersed in a crowded intracellular environment, recent evidence in some metabolic pathways points to the notion that sequential enzymes would be somehow organized in protein assemblies. However, the existence of stable complexes holding a large number of different enzymes by multiple keylock interactions is rather exceptional. Based on fluorescence fluctuation analysis, we have found that protein pairs performing enzymatic steps of glycolysis display strong spatiotemporal coincidence compared to unrelated proteins acting in other pathways. Notably, spatiotemporal coincidence inversely correlated with biochemical distance within the glycolytic pathway. These protein assemblies, which we propose to name protein flocks, display association and dissociation dynamics under the millisecond, well beyond those displayed by membrane-less organelles. To confirm and characterize the compositional specificity of protein flocks, we have applied proximity-labeling and MS assays to glycolysis proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-04 |
| AnnouncementXML | Submission_2026-05-04_04:38:51.267.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Martà Aldea |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-16 09:01:28 | ID requested | |
| ⏵ 1 | 2026-05-04 04:38:52 | announced | |
| 2 | 2026-05-04 23:57:36 | announced | 2026-05-05: Updated project metadata. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: APEX2,glycolysis |
Contact List
| Marti Aldea |
|---|
| contact affiliation | Molecular Biology Institute of Barcelona (IBMB), CSIC, 08028 Barcelona, Catalonia, Spain |
| contact email | mambmc@ibmb.csic.es |
| lab head | |
| Martà Aldea |
|---|
| contact affiliation | IBMB-CSIC |
| contact email | mambmc@ibmb.csic.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065061 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065061
- Label: PRIDE project
- Name: Ultradynamic protein assemblies and network channeling
