PXD065045-1

PXD065045 is an original dataset announced via ProteomeXchange.

Title	Conformational changes in insulin receptor illuminated by live-cell linear dichroism imaging and a FLIP biosensor
Description	Insulin receptor (IR) is a tyrosine kinase involved in vital cellular signaling processes related to glucose metabolism and cell growth. Detailed understanding of molecular mechanisms of IR signaling is important for improved treatments of diabetes and related disorders. Although many aspects of IR signaling have over the past years been elucidated, much of the available information comes from studies made in vitro, using protein preparations. However, observations performed directly in living cells, tissues and organisms are needed for proper understanding of IR signaling. To address this need, we decided to apply the powerful yet underutilized technique of polarization microscopy, which promised to offer a unique opportunity to study interactions of IR with its agonists and antagonists in living cells. Here we present our observations of GP2(1)-eGFP-CD59, a biosensor for polarization-resolved fluorescence microscopy, which allowed us to visualize, in living cells, changes in IR conformation induced by interactions with IR agonists and antagonists, but also, somewhat surprisingly, with kinase inhibitors. Our results open doors to sensitive imaging of various aspects of IR function in living cells, tissues, and ultimately whole organisms, under conditions close to natural.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-31_01:52:01.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tereza Nesporova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	SELECT SERIES Cyclic IMS

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-16 05:30:31	ID requested
⏵ 1	2026-08-31 01:52:01	announced

Dataset with its publication pending

submitter keyword: FLIP biosensors, fluorescence, receptor tyrosine kinase, polarization microscopy,insulin receptor

Frantisek Filandr
contact affiliation	Mass Spectrometry of Biopolymers, Institute of Organic Chemisty and Biochemistry of the CAS, Czech Republic
contact email	frantisek.filandr@uochb.cas.cz
lab head
Tereza Nesporova
contact affiliation	Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic
contact email	tereza.nesporova@uochb.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD065045

PRIDE project URI

• PRIDE
  1. PXD065045
     1. Label: PRIDE project
     2. Name: Conformational changes in insulin receptor illuminated by live-cell linear dichroism imaging and a FLIP biosensor