PXD065042-1

PXD065042 is an original dataset announced via ProteomeXchange.

Title	The late chromatoid body component TSSK2 associates with translational machinery in elongating spermatids in mice
Description	Spermatogenesis culminates in a dramatic morphological transformation, including a tight compaction of the chromatin and nuclear re-shaping that largely silences transcription. Due to transcriptional silencing, the production of the sperm-specific proteins needed for the morphological transformation requires active storage and translational regulation of mRNAs transcribed in earlier cell types. The germline-specific ribonucleoprotein (RNP) granule, the chromatoid body (CB), accumulates RNAs and has a role in RNA storage in early haploid cells (round spermatids). In late haploid cells (elongating spermatids), the CB is transformed to so-called late-CB, whose function in RNA regulation has remained elusive. Here we characterized the function of the late-CB by identifying proteins and RNAs interacting with the known late-CB marker, Testis- Specific Serine Kinase 2 (TSSK2). We showed that TSSK2 interacts with eukaryoti5 translation initiation factors and the late-CB associates with active, localized protein synthesis. Furthermore, we revealed an association of TSSK2 with a specific set of mRNAs that are enriched in polysome fractions in elongating spermatids, supporting the role of the late-CB in the temporally regulated translation. These results link the function of the late-CB to the RNA regulation during late spermatogenesis for the first time, providing important novel information about the RNA regulatory processes required for spermatogenesis and male fertility.
HostingRepository	PRIDE
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-26_17:08:15.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lin Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-16 04:36:18	ID requested
⏵ 1	2025-10-26 17:08:16	announced

10.1530/rep-25-0297;

Lehti MS, Ma L, K, ä, rn, ä S, Laasanen S, Ahmedani A, Olotu O, Bourgery M, Tran P, Sironen A, Kotaja N, The late chromatoid body component TSSK2 is involved in translational regulation in elongating spermatids in mice. Reproduction, 170(6):(2025) [pubmed]

submitter keyword: Spermatogenesis, RNP granules, elongating spermatids, chromatoid body, TSSK2, male fertility, translation

Noora Kotaja
contact affiliation	Integrative Physiology and Pharmacology Unit, Institute of Biomedicine, University of Turku, Turku, Finland.
contact email	nookot@utu.fi
lab head
Lin Ma
contact affiliation	University of Turku
contact email	malin.ml@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD065042

PRIDE project URI

• PRIDE
  1. PXD065042
     1. Label: PRIDE project
     2. Name: The late chromatoid body component TSSK2 associates with translational machinery in elongating spermatids in mice