PXD065039 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics data for - Unravelling the mechanism of selective protein hydrolysis by metal-organic frameworks |
| Description | Metal-organic frameworks (MOFs) are emerging as alternatives to enzymes for selective peptide bond cleavage, which is a crucial procedure in proteomics, protein engineering, and proteolytic drug design. However, the limited understanding behind MOF-assisted protein interactions and hydrolysis mechanisms still severely hinder their development and application. Herein, we explore for the first time the synergy between interactions and reactivity of MOFs towards a whole protein, using both mass spectrometry and molecular docking calculations to understand the cleavage selectivity of UiO-66 towards bovine cytochrome c and hemoglobin and horse myoglobin. Our findings demonstrate that UiO-66 preferentially cleaves peptide bond containing glycine, threonine, and lysine due to the H-bonding and electrostatic interactions between the (positively-charged) “recruiting” residues on the protein surface and linker molecules on the surface of the MOF. This preference is drastically different from the aspartic acid selectivity observed with other Zr-based nanomaterials, and highlights the importance of MOF’s hybrid 3D framework to deliver cleavage specificity in the development of the next generation of MOF-based nanozymes for protein-based fields such as proteomics,biocatalysis and related areas. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-09 |
| AnnouncementXML | Submission_2026-04-09_07:08:32.011.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD065039 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Tamara Vasovic |
| SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-16 02:46:37 | ID requested | |
| ⏵ 1 | 2026-04-09 07:08:32 | announced | |
Publication List
Keyword List
| submitter keyword: cleavage, hydrolysis, LC-MS, protein,MOFs, UiO-66 |
Contact List
| prof. dr |
|---|
| contact affiliation | Full professor at Department of Chemistry, Celestijnenlaan 200f - box 2404 3001 Leuven |
| contact email | tatjana.vogt@kuleuven.be |
| lab head | |
| Tamara Vasovic |
|---|
| contact affiliation | University of Belgade - Faculty of Chemistry |
| contact email | tamara.vasovic@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065039
- Label: PRIDE project
- Name: Proteomics data for - Unravelling the mechanism of selective protein hydrolysis by metal-organic frameworks
