PXD065024-1

PXD065024 is an original dataset announced via ProteomeXchange.

Title	Serum AGP-1-sLex glycoforms report on survivorship of patients with septic shock upon admission to intensive care unit
Description	Septic shock, the excessive host immune response to pathogen infection, poses a major health concern accounting globally for approximately 20% of all deaths. Current methods to establish disease severity are unacceptably slow, unspecific and insensitive, hindering timely and effective treatment and patient care. Aiming to identify easy-to-assay glyco-signatures that may identify and guide the clinical management of the worst affected patients, we applied quantitative glycomics and glycoproteomics to serum longitudinally collected from septic shock survivors (n = 29) and non-survivors (n = 8). Glycomics of all 134 serum samples (2-7 samples/patient, daily sampling until recovery or death) revealed significant N-glycome dynamics in both septic shock survivors and non-survivors. Interestingly, unsupervised clustering of the serum N-glycome upon ICU admission (day 1) indicated survivorship-specific glyco-signatures. We therefore trained a random forest model using the serum N-glycome data from all ICU samples except for day 1 and tested the model using the clinically informative day 1 data. Excitingly, the model accurately classified survivorship outcomes of 35 of 37 patients (specificity 94.6%) and correctly predicted 6 of 8 non-survivors (sensitivity 75%). Targeted N-glycome analysis revealed raised levels of Lewis fucosylation in non-survivors relative to survivors already upon ICU admission. Elevated Lewis fucosylation was recapitulated by comparative glycoproteomics that also identified α-1-acid-glycoprotein as the principal carrier of Lewis fucosylation. Enabled by integrated -omics approaches, this study has laid a foundation for improved clinical management of septic shock patients by uncovering easy-to-assay glyco-signatures that early in the disease course accurately identify individuals with poor survival outcomes.
HostingRepository	PRIDE
AnnounceDate	2025-11-18
AnnouncementXML	Submission_2025-11-17_18:06:25.159.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	The Huong Chau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-14 20:04:26	ID requested
⏵ 1	2025-11-17 18:06:25	announced

10.1016/J.MCPRO.2025.101470;

submitter keyword: alpha-1-acid-glycoprotein, Lewis fucosylation, ICU, glycoproteomics, survivorship,Septic shock, N-glycosylation, glycomics

Morten Thaysen-Andersen
contact affiliation	School of Natural Sciences, Macquarie University, Sydney, Australia
contact email	morten.andersen@mq.edu.au
lab head
The Huong Chau
contact affiliation	Macquarie University
contact email	the-huong.chau@hdr.mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065024
     1. Label: PRIDE project
     2. Name: Serum AGP-1-sLex glycoforms report on survivorship of patients with septic shock upon admission to intensive care unit