⮝ Full datasets listing
PXD064935-1
PXD064935 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of the autoacetyltransferase activity of SnCE1 |
| Description | Gram-negative bacteria use a plethora of virulence factors to infect eukaryotic cells. CE-clan protease-related virulence factors were reported to act as deubiquitinases or deSUMOylases. Some of these enzymes have an additional acetyltransferase activity. The molecular mechanisms underlying this dual activity and the physiological consequences are only marginally understood. Here, we report crystal structures for the Simkania negevensis virulence factor SnCE1 in the apo-state and in a complex with SUMO2. We confirm SnCE1 acting as efficient deSUMOylase and show SnCE1 having an intrinsic autoacetyltransferase activity. Infection assays suggest SnCE1 localizing to the endoplasmic reticulum and mitochondria resulting in fragmented mitochondria. We provide a model for regulation of SnCE1 mediated virulence by lysine acetylation modulating its deSUMOylase activity and cytosolic distribution in the host cell. Our data provide insights into sophisticated regulatory mechanisms used to reprogram bacterial virulence by coordinating the activity of bacterial virulence factors to the metabolic state in the host cell. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-17 |
| AnnouncementXML | Submission_2026-03-17_03:58:13.445.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leif Steil |
| SpeciesList | scientific name: Simkania negevensis; NCBI TaxID: 83561; |
| ModificationList | Acetyl |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-12 06:29:10 | ID requested | |
| ⏵ 1 | 2026-03-17 03:58:13 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: acetylation, bacterial virulence, DatasetType:Proteomics |
Contact List
| Dr. Leif Steil | |
|---|---|
| contact affiliation | Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald |
| contact email | steil@uni-greifswald.de |
| lab head | |
| Leif Steil | |
| contact affiliation | university medicine greifswald |
| contact email | steil@uni-greifswald.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098143/ |
