PXD064933-1

PXD064933 is an original dataset announced via ProteomeXchange.

Title	Integrative structural Interactomics reveals protein organization and structure in a giant virus
Description	Giant viruses are large DNA viruses that infect unicellular and multicellular eukaryotes and form exceptionally large extracellular particles. (Meta)genomics and (meta)transcriptomics have provided insight into their diverse coding repertoire, but many of the proteins remain to be characterized as they lack homology with known proteins. Here, we integrated cross-linking mass spectrometry, quantitative proteomics, computational tools and cryo-EM data to characterize the protein architecture of intact Melbournevirus particles. Based on this, we allocated 88 viral proteins to different virion sub-compartments and proposed topology of 25 inner membrane proteins. We assigned eight components of the capsid in cryo-EM data, including proteins that tether the capsid shell to the membrane, reflecting key points in virion maturation. The data provide a valuable resource and demonstrate the power of an integrative approach to gain system-level structural insights into a poorly characterized biological system.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_12:35:18.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lars Mühlberg
SpeciesList	scientific name: Melbournevirus; NCBI TaxID: NEWT:1560514; scientific name: Acanthamoeba castellanii str. Neff; NCBI TaxID: NEWT:1257118;
ModificationList	ubiquitination signature dipeptidyl lysine; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-12 06:19:17	ID requested
⏵ 1	2026-05-20 12:35:18	announced

Dataset with its publication pending

submitter keyword: cross-linking mass spectrometry, bottom-up proteomics,Giant virus

Fan Liu
contact affiliation	Research Group “Structural Interactomics”, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Roessle-Str. 10 13125 Berlin, Germany
contact email	fliu@fmp-berlin.de
lab head
Lars Mühlberg
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie
contact email	muehlberg@fmp-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064933
     1. Label: PRIDE project
     2. Name: Integrative structural Interactomics reveals protein organization and structure in a giant virus