PXD064898-1

PXD064898 is an original dataset announced via ProteomeXchange.

Title	Proteome Dynamics Underlying Drought Resistance Heterosis in Rice Flag Leaves
Description	This study investigates the molecular mechanisms underlying drought resistance heterosis (DRH) in rice, specifically using the water-saving and drought-resistant rice hybrid Hanyou73 (HY73) and its parental lines Hanhui3 (HH3) and Huhan7B (HH7B). Given the significant impact of drought stress on global food security, improving crop drought resistance is paramount. Our research focused on the proteome-level responses in flag leaves during the critical grain-filling stage under both well-watered (WW) and drought-stressed (DS) conditions. Our primary aim was to unravel the complex proteome dynamics and identify key regulatory pathways that contribute to DRH. Through comprehensive DIA-MS-based proteomic analysis, we sought to pinpoint proteins differentially regulated in response to drought and those exhibiting non-additive effects (NAE) in the hybrid. Specifically, we clarified how proteome remodeling supports enhanced carbon metabolism, identified critical protein post-translational modifications (PTMs) such as lysine acetylation (Kac), and uncovered key metabolic hubs like the "MOS-AcCoA-FA/PR" pathway in conferring HY73's drought resistance advantage. This rich dataset serves as a valuable resource for understanding the proteomic underpinnings of drought adaptation and heterosis in rice, offering promising targets for future crop improvement strategies.
HostingRepository	PRIDE
AnnounceDate	2026-06-28
AnnouncementXML	Submission_2026-06-28_00:58:42.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenbo Cheng
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: NEWT:4530;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 16:38:53	ID requested
⏵ 1	2026-06-28 00:58:42	announced

Ding W, Bi J, Chen S, Wu Z, Liao Z, Huang L, Luo L, Chen L, Zhang X, Integrated multi-omics analysis reveals carbon metabolic reprogramming as a mechanism of drought resistance in hybrid rice. Plant Commun, ():101936(2026) [pubmed]

10.1016/j.xplc.2026.101936;

submitter keyword: DIA-MS, Proteomic, Flag Leaf, Heterosis,Drought Resistance,Rice

Wei Ding
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai,China
contact email	dingw21@m.fudan.edu.cn
lab head
Wenbo Cheng
contact affiliation	Shanghai Omicsspace Bioteh CO., LTD
contact email	wbcheng@omicsspace.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064898

PRIDE project URI

• PRIDE
  1. PXD064898
     1. Label: PRIDE project
     2. Name: Proteome Dynamics Underlying Drought Resistance Heterosis in Rice Flag Leaves