PXD064877-1

PXD064877 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A two-dimensional peptide-centric stability assay maps multidimensional target landscapes unveiling 17Î²-estradiol (E2)-GPX4 binding-induced ferroptosis
Description	We developed a two-dimensional (2D) PEptide-centric Local Stability Assay (PELSA) which allows highly reliable target identification (~ 98% true identification for staurosporine) and systematic readout of multidimensional structural effects (direct interaction, allosteric regulation, complex regulation, etc.) induced by increasing ligand concentrations and local binding rate in native cellular environment. We then demonstrated the versatility of 2D-PELSA by investigating the interactions of 17Î²-estradiol (E2), a predominant sex hormone that exerts a wide spectrum function in both health and disease.
HostingRepository	jPOST
AnnounceDate	2026-06-11
AnnouncementXML	Submission_2026-06-10_08:00:04.781.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanan Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 06:20:47	ID requested
⏵ 1	2026-06-10 08:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: 2D-PELSA, target identification, 17Î²-estradiol, GPX4, ferroptosis, proteome

submitter keyword: 2D-PELSA, target identification, 17Î²-estradiol, GPX4, ferroptosis, proteome

Contact List

Mingliang Ye
lab head
Yanan Li
contact affiliation	Dalian Institute of Chemical Physics
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003862/