PXD064826 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MeCP2 requires interactions with nucleosome linker DNA to read chromatin DNA methylation |
| Description | ethyl-CpG-binding protein 2 (MeCP2) is an epigenetic reader essential for neuronal function, but how it binds DNA methylation within chromatin is still unclear. Using designer nucleosomes we observe that MeCP2 preferentially binds DNA methylation positioned at multiple sites around the nucleosome. Surprisingly, MeCP2 can even bind methylated DNA in bent core nucleosomal DNA. However, this activity requires additional DNA linker interactions, using regions outside of the canonical methyl binding domain. We mapped a novel DNA-binding region in MeCP2 required for this function. Furthermore, we find Histone H1 antagonises the MeCP2-nucleosome interaction through linker binding competition. Overall this reveals that MeCP2 combines nonspecific, but essential, interactions with linker DNA to aid specific binding to nucleosomal methylated DNA, independent of nucleosome structure. Our findings reveal in molecular detail how this clinically important protein interacts with chromatin and they identify novel domain that contributes to full MeCP2 function. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-28_16:27:30.955.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Christos Spanos |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-10 04:53:57 | ID requested | |
| ⏵ 1 | 2026-06-28 16:27:31 | announced | |
Publication List
| Watson JA, Alexander-Howden BK, Hall TS, Wear MA, McGhie F, Clifford G, Wapenaar H, Zou J, Bird A, Wilson MD, MeCP2 requires interactions with nucleosome linker DNA to read chromatin DNA methylation. Nat Commun, 17(1):(2026) [pubmed] |
| 10.1038/s41467-026-71741-0; |
Keyword List
| submitter keyword: MECP2, cross-linking mass spectrometry,DNA methylation, nucleosomes, chromatin |
Contact List
| Dr. Marcus Wilson |
|---|
| contact affiliation | University of Edinburgh |
| contact email | marcus.wilson@ed.ac.uk |
| lab head | |
| Christos Spanos |
|---|
| contact affiliation | University of Edinburgh |
| contact email | spanos.christos@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064826 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064826
- Label: PRIDE project
- Name: MeCP2 requires interactions with nucleosome linker DNA to read chromatin DNA methylation
