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PXD064808-1

PXD064808 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePulldown of RNA binding protein using SARS CoV 2 NSP1 RNA
DescriptionRNA sensing is critical for RLR activation and downstream signaling. To identify molecules that are involved in modulating viral RNA recognition, we utilized biotin-labeled 60 nt single-stranded RNA (ssRNA) from SARS-CoV-2 NSP1 coding region (NSP1 ssRNA) to pull down proteins from RAW264.7 cells. The elutes from biotin-labeled NSP1 ssRNA and control NSP1 ssRNA were subjected to coomassie blue (CBB) staining and liquid chromatography-mass spectrometry (LC-MS). CSDE1, a protein from the cold shock domain protein family, was identified as a potential NSP1 RNA interacting protein.
HostingRepositoryPRIDE
AnnounceDate2026-04-08
AnnouncementXMLSubmission_2026-04-07_20:19:05.350.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYi Zheng
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-06-09 23:53:56ID requested
12026-04-07 20:19:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Pulldown
RNA binding protein
NSP1 RNA
SARS-CoV-2
Contact List
Yi Zheng
contact affiliationShandong University
contact emailzhengyiabc2011@sdu.edu.cn
lab head
Yi Zheng
contact affiliationShandong University
contact emailzhengyiabc2011@sdu.edu.cn
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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